About: SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP

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About: SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP
Creator	Grimm, Dirk Kräusslich, Hans-Georg Schnitzler, Paul Chlanda, Petr Freistaedter, Andrew Börner, Kathleen Müller, Thorsten Anders, Simon Ambiel, Ina Bubeck, Felix Dao, Viet Glass, Bärbel Heuser, Anke-Mareil Khalid, Dina Kirrmaier, Daniel Klein, Steffen Knop, Michael Meurer, Matthias Miranda, Isabel Morales, Ivonne Naumoska, Tamara Schillak, Angelika Sonntag-Buck, Vera Ullrich, Stephanie Winter, Sophie Zimmermann, Liv
Source	MedRxiv
abstract	Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and alternative, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric RT-LAMP using N primers, as well as RT-qPCR using E gene primers showing that the here presented RNA extraction protocol can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot.
has issue date	2020-07-11(xsd:dateTime)
bibo:doi	10.1101/2020.07.08.20147561
has license	medrxiv
sha1sum (hex)	d97806d43a27f248d7993244ed1816c18da1aad7
schema:url	https://doi.org/10.1101/2020.07.08.20147561
resource representing a document's title	SARS-CoV-2 RNA extraction using magnetic beads for rapid large-scale testing by RT-qPCR and RT-LAMP
resource representing a document's body	covid:d97806d43a27f248d7993244ed1816c18da1aad7#body_text
is schema:about of	named entity 'pandemic' named entity 'reagents' named entity 'large' named entity 'pipeline' named entity 'sensitivity' named entity 'pipetting' named entity 'magnetic' named entity 'ONGOING' named entity 'EXTRACTION' named entity 'PRESENCE' named entity 'HIGH THROUGHPUT' named entity 'AS A RESULT OF' named entity 'TESTING' named entity 'LARGE' named entity 'workflow' named entity 'provide' named entity 'result' named entity 'RNA' named entity 'magnetic bead' named entity 'RNA extraction' named entity 'limited' named entity 'kits' named entity 'primers' named entity 'detection' named entity 'throughput' named entity 'Magnetic' named entity 'RNA extraction' named entity 'colorimetric' named entity 'pharyngeal' named entity 'primers' named entity 'RNA extraction' named entity 'quantitative PCR' named entity 'viral RNA' named entity 'RT-qPCR' named entity 'RNA extraction' named entity 'Reverse Transcriptase Enzyme' named entity 'cross contamination' named entity 'medRxiv' named entity 'high-throughput' named entity 'RT-qPCR' named entity 'lysate' named entity 'RNA extraction' named entity 'bead' named entity 'CT 40' named entity 'RNA extraction' named entity 'salts' named entity 'RT-qPCR' named entity 'diagnostic assays' named entity 'buffers' named entity 'RNA' named entity 'RNA' named entity 'medRxiv' named entity 'magnetic field' named entity 'denaturation' named entity 'RNase' named entity 'vaccine' named entity 'RNA' named entity 'IBM' named entity 'sodium citrate' named entity 'PCR'

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