About: Controlled fluorescence quenching by antibody-conjugated graphene oxide to measure tau protein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Controlled fluorescence quenching by antibody-conjugated graphene oxide to measure tau protein
Creator	Li, Weiwei Huang, Ao Ma, Zeyu Shuo, Shi Yao, Tianming Zhang, Luning
Source	Medline; PMC
abstract	We report an ultrasensitive immunoassay for tau protein—a key marker of Alzheimer's disease. This sensing platform relies on graphene oxide (GO) surfaces conjugated with anti-human tau antibody to provide quantitative binding sites for the tau protein. The GO quenches standard fluorescein isothiocyanate labelled tau (tau-FITC) when tau protein and tau-FITC are both present and compete for the binding sites. This change in fluorescence signal can be used to quantitate tau protein. In contrast with traditional enzyme-linked immunosorbent assay (ELISA), our method does not require enzyme-linked secondary antibodies for protein recognition nor does it require an enzyme substrate for optical signal generation. This requires fewer reagents and has less systematic error than the antigen–antibody recognition steps in ELISA. Our method has a tau protein detection limit of 0.14 pmol ml(−1) in buffer. This approach could be developed into a promising biosensor for the detection of tau protein and may be useful in the clinical diagnosis of tau-induced neurodegeneration syndromes.
has issue date	2018-04-11(xsd:dateTime)
bibo:doi	10.1098/rsos.171808
bibo:pmid	29765647
has license	cc-by
sha1sum (hex)	da31b90321637cd3a3464969817540a35d6a597c
schema:url	https://doi.org/10.1098/rsos.171808
resource representing a document's title	Controlled fluorescence quenching by antibody-conjugated graphene oxide to measure tau protein
has PubMed Central identifier	PMC5936912
has PubMed identifier	29765647
schema:publication	R Soc Open Sci
resource representing a document's body	covid:da31b90321637cd3a3464969817540a35d6a597c#body_text
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