About: Important Roles of the C-Terminal Portion of HPC-1/Syntaxin 1A in Membrane Anchoring and Intracellular Localization(1)

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About: Important Roles of the C-Terminal Portion of HPC-1/Syntaxin 1A in Membrane Anchoring and Intracellular Localization(1) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Important Roles of the C-Terminal Portion of HPC-1/Syntaxin 1A in Membrane Anchoring and Intracellular Localization(1)
Creator	Yamamoto, Akitsugu Masaki, Ryuichi Akagawa, Kimio Tashiro, Yutaka
topic	covid:da407f19ed474004613a201e33fc21495021c9ca#this
Source	PMC
abstract	HPC-1/syntaxin 1A (HPC-1), which plays an important role in vesicular transport to the plasma membrane, possesses a hydrophobic sequence at its C terminus. When expressed from cDNA in COS cells, wild-type HPC-1 was localized in the Golgi complex and the plasma membrane. Truncation of the hydrophobic domain resulted in the cytoplasmic localization of the mutant, thus indicating that the domain indeed functions as a membrane anchor. A fusion protein with the C-terminal glycosylation sites was glycosylated in transfected cells, providing evidence that HPC-1 has a transmembrane structure, and that the protein is first inserted into the endoplasmic reticulum and then transported to the plasma membrane. A chimeric protein consisting of Escherichia coli maltose-binding protein with the last 24 amino acids of HPC-1 was inserted into the endoplasmic reticulum in a transmembrane topology and localized along the exocytic pathway of transfected cells similar to HPC-1. These results indicate that the portion is important for intracellular localization of HPC-1.
has issue date	1998-08-01(xsd:dateTime)
bibo:doi	10.1093/oxfordjournals.jbchem.a022113
bibo:pmid	9685720
has license	no-cc
sha1sum (hex)	da407f19ed474004613a201e33fc21495021c9ca
schema:url	https://doi.org/10.1093/oxfordjournals.jbchem.a022113
resource representing a document's title	Important Roles of the C-Terminal Portion of HPC-1/Syntaxin 1A in Membrane Anchoring and Intracellular Localization(1)
has PubMed Central identifier	PMC7109847
has PubMed identifier	9685720
schema:publication	J Biochem
resource representing a document's body	covid:da407f19ed474004613a201e33fc21495021c9ca#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/da407f19ed474004613a201e33fc21495021c9ca
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