About: Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control
Creator	Grimwood, Keith Nissen, Michael Sloots, Theo Bialasiewicz, Seweryn Whiley, David Alsaleh, Asma Lambert, Stephen Ware, Robert
source	Medline; PMC
abstract	BACKGROUND: Carefully conducted, community-based, longitudinal studies are required to gain further understanding of the nature and timing of respiratory viruses causing infections in the population. However, such studies pose unique challenges for field specimen collection, including as we have observed the appearance of mould in some nasal swab specimens. We therefore investigated the impact of sample collection quality and the presence of visible mould in samples upon respiratory virus detection by real-time polymerase chain reaction (PCR) assays. METHODS: Anterior nasal swab samples were collected from infants participating in an ongoing community-based, longitudinal, dynamic birth cohort study. The samples were first collected from each infant shortly after birth and weekly thereafter. They were then mailed to the laboratory where they were catalogued, stored at -80°C and later screened by PCR for 17 respiratory viruses. The quality of specimen collection was assessed by screening for human deoxyribonucleic acid (DNA) using endogenous retrovirus 3 (ERV3). The impact of ERV3 load upon respiratory virus detection and the impact of visible mould observed in a subset of swabs reaching the laboratory upon both ERV3 loads and respiratory virus detection was determined. RESULTS: In total, 4933 nasal swabs were received in the laboratory. ERV3 load in nasal swabs was associated with respiratory virus detection. Reduced respiratory virus detection (odds ratio 0.35; 95% confidence interval 0.27-0.44) was observed in samples where the ERV3 could not be identified. Mould was associated with increased time of samples reaching the laboratory and reduced ERV3 loads and respiratory virus detection. CONCLUSION: Suboptimal sample collection and high levels of visible mould can impact negatively upon sample quality. Quality control measures, including monitoring human DNA loads using ERV3 as a marker for epithelial cell components in samples should be undertaken to optimize the validity of real-time PCR results for respiratory virus investigations in community-based studies.
has issue date	2014-01-09(xsd:dateTime)
bibo:doi	10.1186/1471-2334-14-15
bibo:pmid	24405747
has license	cc-by
sha1sum (hex)	da412bad3772b82d1b3ab2d1aa1e00035869ac55
schema:url	https://doi.org/10.1186/1471-2334-14-15
resource representing a document's title	Nasal swab samples and real-time polymerase chain reaction assays in community-based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control
has PubMed Central identifier	PMC3897990
has PubMed identifier	24405747
schema:publication	BMC Infect Dis
resource representing a document's body	covid:da412bad3772b82d1b3ab2d1aa1e00035869ac55#body_text
is schema:about of	named entity 'swab' named entity 'SAMPLE INTEGRITY' named entity 'TIMING' named entity 'OBSERVED' named entity 'BACKGROUND' covid:arg/da412bad3772b82d1b3ab2d1aa1e00035869ac55 named entity 'NATURE' named entity 'BASED' named entity 'NASAL SWAB' named entity 'RESPIRATORY VIRUSES' named entity 'QUALITY' named entity 'APPEARANCE' named entity 'VIRUS DETECTION' named entity 'LONGITUDINAL STUDIES' named entity 'QUALITY CONTROL' named entity 'UNIQUE' named entity 'GAIN' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'COMMUNITY' named entity 'NASAL SWAB' named entity 'SAMPLES' named entity 'BASED' named entity 'STUDIES' named entity 'UNDERSTANDING' named entity 'PRESENCE OF' named entity 'COMMUNITY' named entity 'POPULATION' named entity 'FIELD' named entity 'INFECTIONS' named entity 'SAMPLE COLLECTION' named entity 'IMPACT' named entity 'REQUIRED' named entity 'VISIBLE' named entity 'SPECIMENS' named entity 'SAMPLES' named entity 'LONGITUDINAL STUDIES' named entity 'CAUSING' named entity 'INCLUDING' named entity 'RESPIRATORY VIRUS' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'HAVE' named entity 'MOULD' named entity 'SPECIMEN COLLECTION' named entity 'POSE' named entity 'CHALLENGES' named entity 'RESPIRATORY VIRUSES' named entity 'INVESTIGATED' named entity 'population' named entity 'samples' named entity 'specimen' named entity 'mould' named entity 'nature' named entity 'real-time polymerase chain reaction' named entity 'longitudinal studies' named entity 'longitudinal studies' named entity 'Nasal swab' named entity 'viruses' named entity 'longitudinal studies' named entity 'significant difference' named entity 'virus' named entity 'adenoviruses' named entity 'virus' named entity 'England' named entity 'rhinovirus' named entity 'virus' named entity 'multivariate analyses' named entity 'PCR' named entity 'pathogenesis' named entity 'human DNA' named entity 'research staff' named entity 'virus' named entity 'supernatant' named entity 'viruses' named entity 'University of Queensland'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software