About: Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media

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About: Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media
Creator	Blaser, Martin Brooks, Andrew Gennaro, Maria Horton, Daniel Panettieri, Reynold Barrett, Emily Bixby, Christian Brooks, ; Carson, Jeffrey Daniel, ; Db, Horton Es, Barrett Horton, B Jagpal, Sugeet Jl, Carson Mj, Blaser Ml, Gennaro Ra, Panettieri Radbel, Jared Roy, Jason Sheldon, Michael Tischfield, Jay Witt, Dana
Source	Elsevier; Medline; PMC; WHO
abstract	As the COVID-19 pandemic sweeps across the world, the availability of viral transport media (VTM) has become severely limited, contributing to delays in diagnosis and rationing of diagnostic testing. Given that SARS-CoV-2 viral RNA has demonstrated stability, we posited that phosphate buffered saline (PBS) may be a viable transport medium, as an alternative to VTM), for clinical qPCR testing. We assessed the intra- and inter-individual reliability of SARS-CoV-2 qPCR in clinical endotracheal secretion samples transported in VTM or PBS, evaluating the stability of the RT-qPCR signal for three viral targets (N gene, ORF1ab, and S gene) when samples were stored in these media at room temperature for up to 18 hours. We report that using PBS as a transport medium has high intra-and inter-individual reliability, maintains viral stability, and is comparable to VTM in the detection of the three SARS-CoV-2 genes through 18 hours of storage. Our study establishes PBS as a clinically useful medium that can be readily deployed for transporting and short-term preservation of specimens containing SARS-CoV-2. Use of PBS as a transport medium has the potential to increase testing capacity for SARS-CoV-2, aiding more widespread screening and early diagnosis of COVID-19.
has issue date	2020-05-13(xsd:dateTime)
bibo:doi	10.1016/j.jmoldx.2020.04.209
bibo:pmid	32405270
has license	no-cc
sha1sum (hex)	da98d42fbc1fd8597aca988834cd5543d934d29b
schema:url	https://doi.org/10.1016/j.jmoldx.2020.04.209
resource representing a document's title	Detection of SARS-CoV-2 is comparable in clinical samples preserved in saline or viral transport media
has PubMed Central identifier	PMC7219422
has PubMed identifier	32405270
schema:publication	J Mol Diagn
resource representing a document's body	covid:da98d42fbc1fd8597aca988834cd5543d934d29b#body_text
is schema:about of	named entity 'gene' named entity 'COVID-19' named entity 'signal' named entity 'qPCR' named entity 'targets' named entity 'sweeps' named entity 'VTM' named entity 'evaluating' named entity 'ASSESSED' named entity 'INTER-' named entity 'HOURS' named entity 'VIRAL TRANSPORT' named entity 'VIRAL' named entity 'AVAILABILITY OF' named entity 'SEVERELY LIMITED' named entity 'EVALUATING' named entity 'samples' named entity 'viral' named entity 'VTM' named entity 'Journal' named entity 'viral RNA' named entity 'diagnostic testing' named entity 'qPCR' named entity 'VTM' named entity 'RT-qPCR' named entity 'qPCR' named entity 'VTM' named entity 'SARS-CoV-2' named entity 'clinical samples' named entity 'SARS-CoV-2' named entity 'VTM' named entity 'PBS' named entity 'VTM' named entity 'SARS-CoV-2' named entity 'acute lung injury' named entity 'disodium hydrogen phosphate' named entity 'VTM' named entity 'RT-qPCR' named entity 'PBS' named entity 'pneumonia' named entity 'hypoxemic' named entity 'qPCR' named entity 'Wuhan' named entity 'clinical laboratory' named entity 'RNA' named entity 'qPCR' named entity 'PBS' named entity 'sars-cov-2' named entity '18 hours' named entity 'VTM' named entity 'molecular diagnostics' named entity 'catheter' named entity 'virus' named entity 'COVID-19' named entity 'public health policy' named entity 'transport medium' named entity 'phosphate buffered saline' named entity 'VTM' named entity 'gene'

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