About: Comparison of eMAG™ versus NucliSENS(®) EasyMAG(®) performance on clinical specimens

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Comparison of eMAG™ versus NucliSENS(®) EasyMAG(®) performance on clinical specimens
Creator	Lévêque, Nicolas Agius, Gérard Beby-Defaux, Agnès Bourgoin, Anne Chessa, Céline Garcia, Magali Giraudeau, Geneviève Plouzeau, Chloé
source	Elsevier; Medline; PMC
abstract	BACKGROUND: eMAG™ (bioMerieux) is a new nucleic acid extraction platform based on magnetic silica technology, like its predecessor, NucliSENS(®) easyMAG(®) (bioMerieux). Using the same reagents and disposables, eMAG™ adds further automation, allowing simultaneous extraction of 48 samples directly from primary tubes, and distribution of nucleic acid extracts on PCR strips or in tubes at the end of the extraction process. OBJECTIVE: To compare the performance of eMAG™ and easyMAG(®) on various clinical specimens. STUDY DESIGN: Respiratory (n = 199), whole blood (n = 50), plasma (n = 25) and urine (n = 25) specimens were extracted in parallel on both platforms. Both qualitative (respiratory virus, cell control, CMV, EBV, HHV6 and BKV detection) and quantitative (respiratory virus and cell control cycle thresolds, and CMV, EBV, HHV6 and BKV viral loads) results were compared. RESULTS: Detection of qualitative targets showed good agreement, ranging from 84.6% for whole blood to 95.9% for respiratory specimens. Correlations between quantitative results were good, with R(2) ranging from 0.802 to 0.995. Quantitative results showed average overall differences below 0.10 log(10) copies/mL between eMAG™ and easyMAG(®). CONCLUSIONS: The two platforms showed comparable performance on the types of clinical specimen tested. With higher automation and throughput than easyMAG(®), the eMAG™ platform is likely to be advantageous for laboratories performing a large number of molecular analyses.
has issue date	2017-01-29(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2017.01.004
bibo:pmid	28160729
has license	no-cc
sha1sum (hex)	da9d9ff02d7cddbd9f73db15cf7a575d8a8f041d
schema:url	https://doi.org/10.1016/j.jcv.2017.01.004
resource representing a document's title	Comparison of eMAG™ versus NucliSENS(®) EasyMAG(®) performance on clinical specimens
has PubMed Central identifier	PMC7185493
has PubMed identifier	28160729
schema:publication	J Clin Virol
resource representing a document's body	covid:da9d9ff02d7cddbd9f73db15cf7a575d8a8f041d#body_text
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