About: Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
Creator	Shen, Hongyuan Chen, Bo Chen, Guang Liu, Ding Tay, Felicia Fang, Shou
Source	Elsevier; Medline; PMC
abstract	Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.
has issue date	2009-05-04(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.04.023
bibo:pmid	19409420
has license	no-cc
sha1sum (hex)	dae1e58fd43fd2ca22fa8851ddc95bdeb84a03dd
schema:url	https://doi.org/10.1016/j.jviromet.2009.04.023
resource representing a document's title	Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
has PubMed Central identifier	PMC7112882
has PubMed identifier	19409420
schema:publication	J Virol Methods
resource representing a document's body	covid:dae1e58fd43fd2ca22fa8851ddc95bdeb84a03dd#body_text
is schema:about of	named entity 'avian' named entity 'genes' named entity 'accommodate' named entity 'firefly luciferase' named entity 'gene' named entity 'expression' covid:arg/dae1e58fd43fd2ca22fa8851ddc95bdeb84a03dd named entity 'gene' named entity 'gene' named entity 'tissue culture' named entity 'gene delivery' named entity 'virus' named entity 'EGFP' named entity 'firefly luciferase' named entity 'mucosal' named entity 'IBV' named entity 'gene' named entity 'mRNA' named entity 'RNA' named entity 'vaccines' named entity 'IBV' named entity 'nucleotide sequence' named entity 'virus' named entity 'EGFP' named entity 'primer' named entity 'protein' named entity 'gene' named entity 'IBV' named entity 'nucleotides' named entity 'IBV' named entity 'IBV' named entity 'gene' named entity 'gene' named entity 'human coronavirus' named entity 'luciferase' named entity 'virus' named entity 'inoculation' named entity 'Hackney' named entity 'gene cassette' named entity 'reporter gene' named entity 'genetic stability' named entity 'cDNA' named entity 'IBV' named entity 'viruses' named entity 'recombinant' named entity 'nucleotides' named entity 'gene' named entity 'protein' named entity 'IBV' named entity 'liver' named entity 'animal species' named entity 'recombinant' named entity 'RT-PCR' named entity 'nucleotides' named entity 'electroporation' named entity 'gene' named entity 'gene' named entity 'liver' named entity 'EGFP' named entity 'vaccines' named entity 'attractive' named entity 'recombinant' named entity 'vaccine' named entity 'EGFP' named entity 'protein' named entity 'genetic stability' named entity 'IBV' named entity 'DMEM' named entity 'virus' named entity 'antigenic' named entity 'lung' named entity 'ultraviolet' named entity 'IBV' named entity 'IBV' named entity 'viruses' named entity 'gene'

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