About: SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallo­graphic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-­terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%.   Goto Sponge  NotDistinct  Permalink

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  • SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallo­graphic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-­terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%.
subject
  • X-ray crystallography
  • Asexual reproduction
  • Viral nonstructural proteins
  • Sarbecovirus
  • Chiroptera-borne diseases
  • Infraspecific virus taxa
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