About: Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation

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About: Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation
Creator	Li, Xiaoying Fu, Lanlan Shen, Wentao Tuo, Decai Yan, Pu
Source	Elsevier; Medline; PMC
abstract	Abstract A novel Rhizobium radiobacter (synonym Agrobacterium tumefaciens)-mediated approach was developed to generate stable infectious clones of plant viruses. This method uses R. radiobacter for both cloning and inoculation of infectious clones, bypassing the requirement of cloning in E. coli to avoid the instability. Only three steps are included in this method: (i) construct viral genome-encoding plasmids in vitro by one-step Gibson assembly; (ii) transform the assembled DNA products into R. radiobacter; (iii) inoculate plants with the R. radiobacter clones containing the viral genome. Stable infectious clones were obtained from two potyviruses papaya ringspot virus (PRSV) and papaya leaf distortion mosaic virus (PLDMV) using this method, whereas attempts utilizing %22classical%22 E. coli cloning system failed repeatedly. This method is simple and efficient, and is promising for a wide application in generation of infectious clones of plant virus, especially for those which are instable in E. coli.
has issue date	2017-10-31(xsd:dateTime)
bibo:doi	10.1016/j.virol.2017.07.012
bibo:pmid	28715654
has license	els-covid
sha1sum (hex)	db8accc961b2c2855300ce4c5db5e214c301be43
schema:url	https://doi.org/10.1016/j.virol.2017.07.012
resource representing a document's title	Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation
has PubMed Central identifier	PMC7173343
has PubMed identifier	28715654
schema:publication	Virology
resource representing a document's body	covid:db8accc961b2c2855300ce4c5db5e214c301be43#body_text
is schema:about of	named entity 'genome' named entity 'Stable' named entity 'inoculate' named entity 'IN VITRO' named entity 'CLONING' named entity 'APPLICATION' named entity 'REQUIREMENT' named entity 'inoculation' named entity 'PRSV' named entity 'plant viruses' named entity 'bypassing' named entity 'clones' named entity 'clones' named entity 'cloning' named entity 'mosaic virus' named entity 'clones' named entity 'plant viruses' named entity 'Rhizobium radiobacter' named entity 'plant viruses' named entity 'GenBank' named entity 'viral genome' named entity 'E. coli' named entity 'inoculation' named entity 'virus' named entity 'plasmids' named entity 'TRIzol' named entity 'wave length' named entity 'PRSV' named entity 'plant viruses' named entity 'helper plasmid' named entity 'plasmid' named entity 'RNA viruses' named entity 'Potyviridae' named entity 'Gibson Assembly' named entity 'E. coli' named entity 'vector' named entity 'DNA polymerase' named entity 'viral genomes' named entity 'promoter sites' named entity 'DNA Polymerases' named entity 'papaya' named entity 'E. coli' named entity 'PRSV' named entity 'PRSV' named entity 'Gibson assembly' named entity 'PCR' named entity 'E. coli' named entity 'plasmids' named entity 'intron' named entity 'E. coli' named entity 'copy number' named entity 'E. coli' named entity 'viral genome' named entity 'pGreen' named entity 'intron' named entity 'PRSV' named entity 'gene' named entity 'flaviviruses' named entity 'plant viruses' named entity 'viral replication' named entity 'PRSV' named entity 'cDNA' named entity 'Hainan' named entity 'viral genome' named entity 'virus' named entity 'plasmid' named entity 'ligation' named entity 'PRSV' named entity 'yeast' named entity 'TaKaRa' named entity 'cloning' named entity 'plant viruses' named entity 'inoculation' named entity 'toxicity' named entity 'PRSV' named entity 'clone' named entity 'copy number'

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