About: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection

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About: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection
Creator	Ben-Ami, Roni Benedek, Gil Fogel, Irit Gertler, Asaf Gurel-Gurevich, Ori Klochendler, Agnes Lavi, Bruno Meshorer, Eran Oiknine-Djian, Esther Rotstein, Zeev Seidel, Matan Sido, Tal Yassour, Moran
Source	MedRxiv
abstract	Testing for active SARS-CoV-2 infection is a fundamental tool in public health measures taken to control the COVID-19 pandemic. Due to the overwhelming use of SARS-CoV-2 RT-PCR tests worldwide, availability of test kits has become a major bottleneck. Here we demonstrate the reliability and efficiency of two simple pooling strategies that can increase testing capacity about 5-fold to 7.5-fold, in populations with a low infection rate. We have implemented the method in a routine clinical diagnosis setting, and already tested 2,168 individuals for SARS-CoV-2 using 311 RNA extraction and RT-PCR kits.
has issue date	2020-04-22(xsd:dateTime)
bibo:doi	10.1101/2020.04.17.20069062
has license	medrxiv
sha1sum (hex)	dc4127b09afc8a09651be49e2ba7e448144d8eb1
schema:url	https://doi.org/10.1101/2020.04.17.20069062
resource representing a document's title	Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection
resource representing a document's body	covid:dc4127b09afc8a09651be49e2ba7e448144d8eb1#body_text
is schema:about of	named entity 'RT-PCR' named entity 'RNA extraction' named entity 'RNA extraction' named entity 'efficiency' named entity 'test kits' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'RNA extraction' named entity 'preprint' named entity 'RT-PCR' named entity 'preprint' named entity 'medRxiv' named entity 'clinical diagnosis' named entity 'RNA' named entity 'CC-BY-NC 4.0' named entity 'lysis' named entity 'CC-BY-NC 4.0' named entity 'probability' named entity 'binary splitting' named entity 'asymptomatic' named entity 'global COVID-19 pandemic' named entity 'PCR' named entity 'social distancing' named entity 'RT-PCR' named entity 'COVID' named entity 'clinical cases' named entity 'preprint' named entity 'RNA' named entity 'lysate' named entity 'preprint' named entity 'COVID' named entity 'viral genome' named entity 'diagnostic methods' named entity 'preprint' named entity 'alternative methods' named entity 'public health interventions' named entity 'RNA extraction' named entity 'RT-PCR' named entity 'false positive and false negative' named entity 'RNA' named entity 'PCR-based methods' named entity 'nasopharyngeal swab' named entity 'COVID' named entity 'preprint' named entity 'lysis buffer' named entity 'reverse transcription' named entity 'preprint' named entity 'group testing' named entity 'group testing' named entity 'PCR based' named entity 'BGI' named entity 'SARS-CoV-2' named entity 'preprint' named entity 'SARS-CoV' named entity 'RNA extraction' named entity 'RNA extraction' named entity 'false positives' named entity 'preprint' named entity 'BGI' named entity 'Proteinase' named entity 'Nasopharyngeal swab' named entity 'COVID' named entity 'independent and identically distributed' named entity 'SARS-CoV-2' named entity 'RNA extraction' named entity 'COVID-19' named entity 'asymptomatic' named entity 'asymptomatic' named entity 'pre-test probability' named entity 'CC-BY-NC 4.0' named entity 'Fluorescent' named entity 'CC-BY-NC 4.0' named entity 'RNA extraction'

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