About: The importance of the immunodominant CD8+ T cell epitope of Plasmodium berghei circumsporozoite protein in parasite- and vaccine-induced protection

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About: The importance of the immunodominant CD8+ T cell epitope of Plasmodium berghei circumsporozoite protein in parasite- and vaccine-induced protection Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The importance of the immunodominant CD8+ T cell epitope of Plasmodium berghei circumsporozoite protein in parasite- and vaccine-induced protection
Creator	Reyes-Sandoval, Arturo Silvie, Olivier Matuschewski, Kai Bauza, Karolis Gibbins, Matthew Glover, Maya Hafalla, Clemence Liu, Jasmine Müller, Katja Putrianti, Elyzana
Source	BioRxiv
abstract	The circumsporozoite protein (CSP) builds up the surface coat of sporozoites and is the leading malaria pre-erythrocytic-stage vaccine candidate. CSP has been shown to induce robust CD8+ T cell responses that are capable of eliminating developing parasites in hepatocytes resulting in protective immunity. In this study, we characterised the importance of the immunodominant CSP-derived epitope, SYIPSAEKI, of Plasmodium berghei in both sporozoite- and vaccine-induced protection in murine infection models. In BALB/c mice, where SYIPSAEKI is efficiently presented in the context of the major histocompatibility complex class I (MHC-I) molecule H-2-Kd, we established that epitope-specific CD8+ T cell responses contribute to parasite killing following sporozoite immunisation. Yet, sterile protection was achieved in the absence of this epitope substantiating the concept that other antigens can be sufficient for parasite-induced protective immunity. Furthermore, we demonstrated that SYIPSAEKI-specific CD8+ T cell responses elicited by viral-vectored CSP-expressing vaccines effectively targeted parasites in hepatocytes. The resulting sterile protection strictly relied on the expression of SYIPSAEKI. In C57BL/6 mice, which are unable to present the immunodominant epitope, CSP-based vaccines did not confer complete protection, despite the induction of high levels of CSP-specific antibodies. These findings underscore the significance of CSP in protection against malaria pre-erythrocytic stages and demonstrate that a significant proportion of the protection against the parasite is mediated by CD8+ T cells specific for the immunodominant CSP-derived epitope.
has issue date	2020-04-05(xsd:dateTime)
bibo:doi	10.1101/2020.04.03.024539
has license	biorxiv
sha1sum (hex)	dc9c5e682172d11c94a6c67e33c3af1a63cecf0e
schema:url	https://doi.org/10.1101/2020.04.03.024539
resource representing a document's title	The importance of the immunodominant CD8+ T cell epitope of Plasmodium berghei circumsporozoite protein in parasite- and vaccine-induced protection
schema:publication	bioRxiv
resource representing a document's body	covid:dc9c5e682172d11c94a6c67e33c3af1a63cecf0e#body_text
is schema:about of	covid:arg/dc9c5e682172d11c94a6c67e33c3af1a63cecf0e named entity 'vaccine' named entity 'coat' named entity 'sporozoites' named entity 'circumsporozoite protein' named entity 'parasites' named entity 'hepatocytes' named entity 'CD8+' named entity 'CD8+ T cell' named entity 'immunisation' named entity 'vaccines' named entity 'epitope' named entity 'sporozoites' named entity 'CSP' named entity 'sporozoite' named entity 'immunised' named entity 'parasite' named entity 'TRIzol' named entity 'coat protein' named entity 'staining' named entity 'RNA' named entity 'orders of magnitude' named entity 'CSP' named entity 'IFN-g' named entity 'RTS,S' named entity 'parasites' named entity 'peptide' named entity 'MHC-I' named entity 'intracellular' named entity 'CD11a' named entity 'incubation' named entity 'live attenuated' named entity 'staining' named entity 'immunological studies' named entity 'CD8+ T cell' named entity 'BALB/c' named entity 'CSP' named entity 'sporozoite' named entity 'CSP' named entity 'BALB/c' named entity 'immunodominant' named entity 'vaccination' named entity 'heparin' named entity 'infection' named entity 'C57BL/6' named entity 'assay' named entity 'parasite' named entity 'flow cytometry' named entity 'Trypan Blue' named entity 'mice' named entity 'serum' named entity 'staining' named entity 'sporozoites' named entity 'capillary' named entity 'blood cells' named entity 'immunodominant' named entity 'CD8+ T cell' named entity 'vaccine' named entity 'mice' named entity 'CD8+ T cells' named entity 'hepatocytes' named entity 'parasite' named entity 'parasite' named entity 'cDNA' named entity 'mice' named entity 'CSP' named entity 'acetone' named entity 'CD8' named entity 'mice' named entity 'Immunofluorescent' named entity 'malaria vaccine' named entity 'sporozoite' named entity 'CD8+ T cell' named entity 'tail vein' named entity 'fluorescently'

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