About: Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

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About: Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
Creator	Bae, Hi-Gung Nitsche, Andreas Radonić, Aleksandar Siegert, Wolfgang Thulke, Stefanie Müller, Marcel
Source	Medline; PMC
abstract	Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
has issue date	2005-02-10(xsd:dateTime)
bibo:doi	10.1186/1743-422x-2-7
bibo:pmid	15705200
has license	cc-by
sha1sum (hex)	dce8be353f8ed76957af86118e1b77fd82f54292
schema:url	https://doi.org/10.1186/1743-422x-2-7
resource representing a document's title	Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
has PubMed Central identifier	PMC549079
has PubMed identifier	15705200
schema:publication	Virol J
resource representing a document's body	covid:dce8be353f8ed76957af86118e1b77fd82f54292#body_text
is schema:about of	named entity 'tested' named entity 'genes' named entity 'Human' named entity 'Herpesvirus' named entity 'potential' named entity 'JOURNAL ' named entity 'REFERENCE GENE' named entity 'CAMELPOX VIRUS' named entity 'CELLS' named entity 'CRITERIA' named entity 'POTENTIAL' named entity 'PEPTIDYL' named entity 'REPORTED' named entity 'STUDIES' named entity 'THESE' named entity 'INFECTED' named entity 'REFERENCE GENE' named entity 'PROLYL' named entity 'RANKING' named entity 'YELLOW FEVER VIRUS' named entity 'REFERENCE' named entity 'TOOL' named entity 'EXPRESSION' named entity 'COMPARED' named entity 'DEPENDENT' named entity 'GENES' named entity 'EXPERIMENTS' named entity 'STABLE' named entity 'SARS' named entity 'SELECTION' named entity 'PCR ANALYSIS' named entity 'THEIR' named entity 'HUMAN HERPESVIRUS' named entity 'VIRUS' named entity 'CAMELPOX VIRUS' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'REAL-TIME PCR' named entity 'CELLS' named entity 'VIRUS' named entity 'INFECTED' named entity 'YELLOW FEVER VIRUS' named entity 'REAL-TIME PCR ' named entity 'CORONA' named entity 'HUMAN HERPESVIRUS' named entity 'CELLULAR' named entity 'RANKED' named entity 'CYTOMEGALOVIRUS' named entity 'TESTED' named entity 'HUMAN CELL LINES' named entity 'MRNA EXPRESSION' named entity 'ACTIN' named entity 'CYTOMEGALOVIRUS INFECTIONS' named entity 'LEVELS' named entity 'INVESTIGATING' named entity 'DETERMINED BY' named entity 'SARS CORONAVIRUS' named entity 'VIROLOGY' named entity 'PROTEIN ' named entity 'ISOMERASE' named entity 'VIRAL REPLICATION' named entity 'TATA-BOX BINDING PROTEIN' named entity 'USE' named entity 'PREVIOUSLY' named entity 'reference' named entity 'Cytomegalovirus' named entity 'viral replication' named entity 'gene' named entity 'Ten' named entity 'reference' named entity 'reference genes' named entity 'virus' named entity 'mRNA expression' named entity 'real-time PCR' named entity 'Cytomegalovirus' named entity 'Human Herpesvirus-6' named entity 'Yellow fever virus'

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