About: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform

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About: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
Creator	Park, Su-Jin Song, Min-Suk Choi, Won-Suk Chun, Sungkun Kim, Eun-Ha Si, Young-Jae Ahn, Su Antigua, C Choi, Young Hee Baek, Yun Hong, Seung Jeong, Ju Joy, Khristine Kaith, Khristine Kim, Young-Il Kwon, Hyeok-Il Lloren, S Shin, Kyeong
Source	Medline; PMC
abstract	BACKGROUND: In addition to seasonal influenza viruses recently circulating in humans, avian influenza viruses (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged and demonstrated human infection abilities with high mortality rates. Although influenza viral infections are usually diagnosed using viral isolation and serological/molecular analyses, the cost, accessibility, and availability of these methods may limit their utility in various settings. The objective of this study was to develop and optimized a multiplex detection system for most influenza viruses currently infecting humans. METHODS: We developed and optimized a multiplex detection system for most influenza viruses currently infecting humans including two type B (both Victoria lineages and Yamagata lineages), H1N1, H3N2, H5N1, H5N6, and H7N9 using Reverse Transcriptional Loop-mediated Isothermal Amplification (RT-LAMP) technology coupled with a one-pot colorimetric visualization system to facilitate direct determination of results without additional steps. We also evaluated this multiplex RT-LAMP for clinical use using a total of 135 clinical and spiked samples (91 influenza viruses and 44 other human infectious viruses). RESULTS: We achieved rapid detection of seasonal influenza viruses (H1N1, H3N2, and Type B) and avian influenza viruses (H5N1, H5N6, H5N8 and H7N9) within an hour. The assay could detect influenza viruses with high sensitivity (i.e., from 100 to 0.1 viral genome copies), comparable to conventional RT-PCR-based approaches which would typically take several hours and require expensive equipment. This assay was capable of specifically detecting each influenza virus (Type B, H1N1, H3N2, H5N1, H5N6, H5N8 and H7N9) without cross-reactivity with other subtypes of AIVs or other human infectious viruses. Furthermore, 91 clinical and spiked samples confirmed by qRT-PCR were also detected by this multiplex RT-LAMP with 98.9% agreement. It was more sensitive than one-step RT-PCR approach (92.3%). CONCLUSIONS: Results of this study suggest that our multiplex RT-LAMP assay may provide a rapid, sensitive, cost-effective, and reliable diagnostic method for identifying recent influenza viruses infecting humans, especially in locations without access to large platforms or sophisticated equipment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12879-019-4277-8) contains supplementary material, which is available to authorized users.
has issue date	2019-08-01(xsd:dateTime)
bibo:doi	10.1186/s12879-019-4277-8
bibo:pmid	31370782
has license	cc-by
sha1sum (hex)	dd12c39ca963dca8336d7f30c8842d892ec8236c
schema:url	https://doi.org/10.1186/s12879-019-4277-8
resource representing a document's title	Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
has PubMed Central identifier	PMC6669974
has PubMed identifier	31370782
schema:publication	BMC Infect Dis
resource representing a document's body	covid:dd12c39ca963dca8336d7f30c8842d892ec8236c#body_text
is schema:about of	named entity 'influenza' named entity 'viruses' named entity 'amplification' named entity 'isothermal' named entity 'colorimetric detection' named entity 'viral' named entity 'human' named entity 'isolation' named entity 'influenza' named entity 'demonstrated' named entity 'high' named entity 'H7N9' named entity 'viruses' named entity 'infecting humans' named entity 'SYBR' named entity 'chicken' named entity 'Qiagen' named entity 'influenza' named entity 'viruses' named entity 'viral particles' named entity 'diagnostic tools' named entity 'RT-PCR' named entity 'infecting humans' named entity 'serology' named entity 'virus' named entity 'denaturation' named entity 'H5N1' named entity 'viral infections' named entity 'developing countries' named entity 'colorimetric methods' named entity 'RT-PCR' named entity 'viruses' named entity 'H7N9' named entity 'PDF' named entity 'viruses' named entity 'human influenza' named entity 'incubator' named entity 'seasonal flu' named entity 'polymerase' named entity 'RT-PCR' named entity 'viruses' named entity 'viral RNA' named entity 'Loop-mediated isothermal amplification' named entity 'viral genome' named entity 'virus' named entity 'microliter' named entity 'H3N2' named entity 'pH indicator' named entity 'viral RNA' named entity 'infecting humans' named entity 'virus' named entity 'highly pathogenic avian influenza' named entity 'influenza' named entity 'H5N8' named entity 'influenza viruses' named entity 'viruses' named entity 'H5N6' named entity 'infection' named entity 'syringe' named entity 'qRT-PCR' named entity 'viruses' named entity 'reverse transcription' named entity 'diagnostic tool' named entity 'avian influenza viruses'

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