About: A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses

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About: A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses
Creator	Chen, Yin Chen, Zhong-Wen Guo, Jin-Lei He, Pei-Yan Lei, Yong-Liang Luo, Jian-Yong Wang, Heng-Hui Yan, Yong Zhu, Guo-Ying
Source	Medline; PMC
abstract	We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). Performance of rMLA was evaluated by comparing with real-time RT-PCR. Detection data from clinical specimens showed that the rMLA had diagnostic sensitivities of 97.10% for FluA, 94.59% for FluB, 98.68% for PIV-3, 94.87% for RSV and 95.92% for MPV (No Data for MERS-CoV due to the lack of positive specimens). Data of analytical sensitivities showed that the detection limits of the rMLA assay were 5–25 viral RNA copies per μl for FluA, FluB, PIV-3 and MERS-CoV, approximate to the real-time RT-PCR assay; while the values were 8 and 22copies/μl for MPV and RSV, lower than the real-time RT-PCR(78 and 114 copies/μl respectively). The results indicated that the rMLA is a sensitive, specific detection tool and comparable to real-time RT-PCR, especially suitable for high-throughput detection of respiratory specimens.
has issue date	2017-06-17(xsd:dateTime)
bibo:doi	10.18632/oncotarget.18533
bibo:pmid	29228581
has license	cc-by
sha1sum (hex)	dd3c5f3af25e4c6fe5395625ed96d9244cd467b9
schema:url	https://doi.org/10.18632/oncotarget.18533
resource representing a document's title	A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses
has PubMed Central identifier	PMC5722533
has PubMed identifier	29228581
schema:publication	Oncotarget
resource representing a document's body	covid:dd3c5f3af25e4c6fe5395625ed96d9244cd467b9#body_text
is schema:about of	named entity 'PCR' named entity 'CHIP ASSAY' named entity 'RESPIRATORY' named entity 'technology' named entity 'develop' named entity 'TECHNOLOGY' covid:arg/dd3c5f3af25e4c6fe5395625ed96d9244cd467b9 named entity 'LUMINEX' named entity 'MULTIPLEX' named entity 'RESPIRATORY VIRUSES' named entity 'MULTIPLEX' named entity 'BASED' named entity 'DETECTION' named entity 'CHIP ASSAY' named entity 'LIQUID' named entity 'COMMON' named entity 'LUMINEX' named entity 'STEP' named entity 'TECHNOLOGY' named entity 'REVERSE TRANSCRIPTION' named entity 'LIQUID' named entity 'SIMULTANEOUS' named entity 'multiplex' named entity 'assay' named entity 'rMLA' named entity 'rMLA' named entity 'Luminex' named entity 'real-time RT-PCR' named entity 'MPV' named entity 'RSV' named entity 'real-time RT-PCR' named entity 'hybridization' named entity 'thermocycler' named entity 'Biotech' named entity 'standard curve' named entity 'analyte' named entity 'viruses' named entity 'real-time PCR' named entity 'ethical standards' named entity 'Korea' named entity 'rMLA' named entity 'SPSS' named entity 'MERS-CoV' named entity 'hybridization' named entity 'real-time RT-PCR' named entity 'phycoerythrin' named entity 'World Health Organization' named entity 'primers' named entity 'viruses' named entity 'sensitivity and specificity' named entity 'MERS-CoV' named entity 'hybridization' named entity 'fluorescent' named entity 'rMLA' named entity 'US FDA' named entity 'Luminex' named entity 'Bio-Rad Laboratories' named entity 'nucleotide' named entity 'oncotarget' named entity 'MERS-CoV' named entity 'Jiaxing' named entity 'virus' named entity 'MERS-CoV' named entity 'nasopharyngeal swabs' named entity 'primers' named entity 'RNA transcripts' named entity 'real-time PCR' named entity 'multi-target' named entity 'adverse interactions' named entity 'microspheres' named entity 'viral RNA' named entity 'RSV' named entity 'Shanghai'

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