About: Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation
Creator	Bhadra, Sanchita Choi, Joon Ellington, Andrew Sullivan, Christopher Ragan, Katherine Towers, Dalton
Source	MedRxiv; Medline
abstract	Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored SARS-CoV-2 RNA from Asuragen as an alternative diagnostic standard to ATCC genomic SARS-CoV-2 RNA and heat inactivated virions and provide guidelines for its use in RT-qPCR.
has issue date	2020-08-04(xsd:dateTime)
bibo:doi	10.1101/2020.08.01.20166173
bibo:pmid	32793925
has license	medrxiv
sha1sum (hex)	dd3e9d2b7758e7c89ed849c94c789b18368619f5
schema:url	https://doi.org/10.1101/2020.08.01.20166173
resource representing a document's title	Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation
has PubMed identifier	32793925
schema:publication	medRxiv : the preprint server for health sciences
resource representing a document's body	covid:dd3e9d2b7758e7c89ed849c94c789b18368619f5#body_text
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