About: High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice

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About: High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice
Creator	Kim, Hyun Hyouk, • Jae, Youm Jeon, Heung Jeong, Joong Kim, Hee Kim, Sun Mi, Min Ran, Sung Won, Joung Won, Jung
Source	Medline; PMC
abstract	Plastid transformation has to date been applied to the expression of heterologous genes involved in agronomic traits and to the production of useful recombinant proteins. Here, we report a feasibility study for producing the human β-site APP cleaving enzyme (BACE) via transformation of tobacco chloroplasts. Stable integration of human BACE into the plastome was confirmed by PCR. Genomic Southern blot analysis detected the presence of the tobacco aadA and human BACE genes between trnI and trnA in the plastome. Northern blot analysis revealed that the aadA and BACE genes were both properly transcribed into a dicistronic transcriptional unit. Human BACE protein expression in transplastomic tobacco was determined by western blot analysis. ELISA analysis revealed that, based on a dilution series of E. coli-derived BACE as a standard, transplastomic lines accumulated BACE to levels of 2.0% of total soluble proteins. When mice were gavaged with the transplastomic tobacco extracts, they showed an immune response against the BACE antigen. The successful production of plastid-based BACE protein has the potential for developing a plant-based vaccine against Alzheimer disease.
has issue date	2010-03-15(xsd:dateTime)
bibo:doi	10.1007/s11248-010-9383-8
bibo:pmid	20229285
has license	no-cc
sha1sum (hex)	dd63c8a6ff3ae72819472ca9eef5dfb0f13afb5e
schema:url	https://doi.org/10.1007/s11248-010-9383-8
resource representing a document's title	High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice
has PubMed Central identifier	PMC7089353
has PubMed identifier	20229285
schema:publication	Transgenic Res
resource representing a document's body	covid:dd63c8a6ff3ae72819472ca9eef5dfb0f13afb5e#body_text
is schema:about of	named entity 'vaccine' named entity 'properly' named entity 'mice' named entity 'cleaving' named entity 'expression' named entity 'tobacco' named entity 'enzyme' named entity 'HIGH' named entity 'PCR' named entity 'ALZHEIMER DISEASE' named entity 'ELISA' named entity 'HUMAN' named entity 'DERIVED' named entity 'SOUTHERN BLOT ANALYSIS' named entity 'SOLUBLE' named entity 'ANTIGEN' named entity 'APPLIED' named entity 'PROTEIN ' named entity 'PLANT-' named entity 'PLASTID' named entity 'PROTEINS' named entity 'PRESENCE OF' named entity 'EXTRACTS' named entity 'IMMUNE RESPONSE' named entity 'WESTERN BLOT ANALYSIS' named entity 'TRANSCRIBED' named entity 'CHLOROPLASTS' named entity 'POTENTIAL' named entity 'UNIT' named entity 'CONFIRMED BY' named entity 'E. COLI' named entity 'LINES' named entity 'DATE' named entity 'USEFUL' named entity 'ANALYSIS' named entity 'EXPRESSION' named entity 'ACCUMULATED' named entity 'TRNA' named entity 'SERIES' named entity 'STABLE' named entity 'PRODUCTION' named entity 'ENZYME' named entity 'PRODUCING' named entity 'TRANSFORMATION' named entity 'PLASTID TRANSFORMATION' named entity 'AADA' named entity 'INTEGRATION' named entity 'REVEALED' named entity 'PROTEIN EXPRESSION' named entity 'REPORT' named entity 'RECOMBINANT PROTEINS' named entity 'MICE' named entity 'TOBACCO' named entity 'TRANSCRIPTIONAL' named entity 'TRANSGENIC TOBACCO' named entity 'APP' named entity 'CHLOROPLASTS' named entity 'ENZYME' named entity 'IMMUNOGENICITY' named entity 'SITE' named entity 'BACE' named entity 'DETECTED' named entity 'APP' named entity 'DILUTION' named entity 'GENOMIC' named entity 'DEVELOPING' named entity 'TRNI' named entity 'LEVELS' named entity 'STANDARD' named entity 'HETEROLOGOUS' named entity 'INVOLVED'

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