About: Detection of yellow head virus in shrimp by loop-mediated isothermal amplification (LAMP)

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of yellow head virus in shrimp by loop-mediated isothermal amplification (LAMP)
Creator	Savan, Ram Itami, Toshiaki Kono, Tomoya Mekata, Tohru Sakai, Masahiro Kasornchandra, Jiraporn Yoshida, Terutoyo
Source	Elsevier; Medline; PMC
abstract	Abstract Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the structural glycoprotein gene of yellow head virus (YHV). The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, sensitivity and rapidity under isothermal conditions with a set of four specially designed primers that recognize six distinct sequences of the target. The whole procedure is very simple and rapid, and reaction time and temperatures were optimized for 60min at 65°C, respectively. Detection of gene amplification could be accomplished by agarose gel electrophoresis. The standardized RT-LAMP procedure was used to detect YHV in the heart and gill from infected shrimp. Thus, the RT-LAMP assay is extremely rapid, cost-effective, sensitive and specific and has potential usefulness for rapid diagnosis for YHV detection in shrimp.
has issue date	2006-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.02.012
bibo:pmid	16597466
has license	els-covid
sha1sum (hex)	de6d9a0d9734988389599ea7731f23ee8b3a23ed
schema:url	https://doi.org/10.1016/j.jviromet.2006.02.012
resource representing a document's title	Detection of yellow head virus in shrimp by loop-mediated isothermal amplification (LAMP)
has PubMed Central identifier	PMC7172593
has PubMed identifier	16597466
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:de6d9a0d9734988389599ea7731f23ee8b3a23ed#body_text
is schema:about of	named entity 'shrimp' named entity 'conditions' named entity 'shrimp' named entity 'LAMP' named entity 'rapidity' named entity 'Thus' named entity 'assay' named entity 'specificity' named entity 'agarose gel electrophoresis' named entity 'temperatures' named entity 'gene' named entity 'agarose gel electrophoresis' named entity 'isothermal conditions' named entity 'yellow head' named entity 'loop-mediated isothermal amplification' named entity 'glycoprotein' named entity 'RNA' named entity 'WSDV' named entity 'loop-mediated isothermal amplification' named entity 'cross-reaction' named entity 'lysis buffer' named entity 'DNA polymerase' named entity 'genome' named entity 'Enzyme' named entity 'primers' named entity 'WSDV' named entity 'PCR' named entity 'YHV' named entity 'primers' named entity 'nucleocapsid' named entity 'nested RT-PCR' named entity 'gene' named entity 'single-stranded RNA' named entity 'reaction time' named entity 'molecular size' named entity 'RNA' named entity 'PCR' named entity 'WSDV' named entity 'isothermal' named entity 'confirmatory diagnosis' named entity '2.0' named entity 'primers' named entity 'sensitivity and specificity' named entity 'RNA' named entity 'RNAs' named entity 'Enzyme' named entity 'YHV' named entity 'genome' named entity 'YHV' named entity 'RNA' named entity 'DNA polymerase' named entity 'virus' named entity 'reverse transcriptase' named entity 'glycoprotein' named entity 'electrophoresed' named entity 'Eiken Chemical' named entity 'cross-reaction' named entity 'thermal cycler' named entity 'WSDV' named entity 'YHV' named entity 'YHV' named entity 'molecular size' named entity 'extraction of RNA' named entity 'RNA extraction' named entity 'RNA' named entity 'GAV' named entity 'nested PCR' named entity 'rod-shaped' named entity 'RNA' named entity 'gene' named entity 'distilled water' named entity 'reaction time' named entity 'YHV' named entity 'RT-PCR' named entity 'YHV'

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