About: Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification

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About: Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification
Creator	Nishimura, Hidekazu Shirato, Kazuya Saijo, Masayuki Okamoto, Michiko Tashiro, Masato Taguchi, Fumihiro Noda, Masahiro
Source	Elsevier; Medline; PMC
abstract	Human respiratory syncytial virus (RSV) is a major causative agent of lower respiratory tract infections in children and the elderly. A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was developed assay to amplify the genome of RSV subgroups A and B, in order to improve current diagnostic methods for RSV infection. The primer sets for RT-LAMP were designed using highly conserved nucleotide sequences in the matrix protein region of subgroups A and B, and were specific for each subgroup. The RT-LAMP efficiency was compared to virus isolation and a commercially available enzyme immunoassay (EIA) for RSV detection (BD Directigen EZ RSV test™), using nasopharyngeal aspirates from 59 children with respiratory tract infections. The RT-LAMP was specific for RSV and could not detect other respiratory pathogens. 61% (36/59) of children were positive by RT-LAMP, 34% (20/59) by viral isolation, and 56% (26/46) by EZ RSV. Of 16 specimens that were negative by both antigen detection and virus isolation, 12.5% (2/16) were RT-LAMP positive. These results suggest that the RT-LAMP is more sensitive than other methods used to detect RSV. The RT-LAMP assay developed in this study may be useful for diagnostic and epidemiological studies of RSV infection.
has issue date	2006-10-18(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.09.014
bibo:pmid	17052763
has license	no-cc
sha1sum (hex)	ded7d3ae6f8bbe7866a224433f9aac0471e758ae
schema:url	https://doi.org/10.1016/j.jviromet.2006.09.014
resource representing a document's title	Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification
has PubMed Central identifier	PMC7112771
has PubMed identifier	17052763
schema:publication	J Virol Methods
resource representing a document's body	covid:ded7d3ae6f8bbe7866a224433f9aac0471e758ae#body_text
is schema:about of	named entity 'REVERSE TRANSCRIPTION' named entity 'ANTIGEN DETECTION' named entity 'LAMP' named entity 'REVERSE TRANSCRIPTION' named entity 'TO DETECT' named entity 'SUBGROUP' named entity 'SENSITIVE' named entity 'COMPARED' named entity 'USING' named entity 'current' named entity 'infection' named entity 'human respiratory syncytial virus' named entity 'DIAGNOSIS' named entity 'ASSAY' named entity 'CAUSATIVE AGENT' named entity 'IS A' named entity 'VIRUS ISOLATION' named entity 'SPECIMENS' named entity 'RSV INFECTION' named entity 'STUDY' named entity 'CHILDREN' named entity 'PROTEIN REGION' named entity 'ELDERLY' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' named entity 'DIAGNOSTIC' named entity 'RSV' named entity 'A MAJOR' named entity 'RSV TEST' named entity 'VIRAL ISOLATION' named entity '282' named entity 'NASOPHARYNGEAL' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'NEGATIVE' named entity 'ENZYME IMMUNOASSAY' named entity 'PRIMER' named entity 'CURRENT' named entity 'NUCLEOTIDE SEQUENCES' named entity 'DETECT' named entity 'LOWER RESPIRATORY TRACT INFECTIONS' named entity 'MAY BE' named entity 'USED' named entity 'RT-LAMP ASSAY' named entity 'HIGHLY' named entity 'EFFICIENCY' named entity 'USEFUL' named entity 'EPIDEMIOLOGICAL STUDIES' named entity 'GENOME' named entity 'CONSERVED' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS INFECTION' named entity 'USING' named entity 'DETECTION' named entity 'ASPIRATES' named entity 'SPECIFIC' named entity 'MATRIX PROTEIN' named entity 'SUBGROUPS' named entity 'THESE' named entity 'AVAILABLE' named entity 'RESPIRATORY PATHOGENS' named entity '12.5' named entity 'METHODS' named entity 'RESULTS' named entity 'POSITIVE' named entity 'AMPLIFY' named entity 'SUGGEST' named entity 'RESPIRATORY TRACT INFECTIONS' named entity 'ORDER' covid:arg/ded7d3ae6f8bbe7866a224433f9aac0471e758ae named entity 'methods' named entity 'region' named entity 'subgroups' named entity 'assay' named entity 'amplification' named entity 'These' named entity 'detect'

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