About: New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification

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About: New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification
Creator	Zhang, Chiyu Liu, Jia Wang, Lili Lan, Ke Cui, Meng Chen, Qianqian He, Zhixiang Mu, Yonglin Xiao, Ming Yao, Fujia Zeng, Jiawei
Source	Elsevier; Medline; PMC
abstract	Abstract Human respiratory syncytial virus (HRSV) is a seasonal respiratory pathogen that causes respiratory infection in children and the elderly. A new, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid (within 1h), simultaneous detection of A and B group HRSV. Primers specific for groups A and B were designed to amplify the N and L genes of HRSV, respectively. A fluorescent dye, calcein, was used as an indicator for the endpoint visual detection and/or real-time amplification of HRSV RNA. The detection limit of the new method was 281.17 50% tissue culture infective doses (TCID50)/ml for HRSV A and 1.58 TCID50/ml for HRSV B. To evaluate the validity of this method, a comparison with RT-PCR was performed using 77 nasopharyngeal swabs as samples. Both RT-LAMP and RT-PCR detected HRSV in 38 HRSV samples, yielding a positive rate of 49%. Of the RT-LAMP positive samples, 36 (95%) were also positive by RT-PCR, while two were negative by RT-PCR. Among the 36 RT-LAMP and RT-PCR positive samples, 11 belonged to HRSV group A, while 25 belonged to group B. The results show that the new RT-LAMP is simple, rapid and well suited for HRSV diagnosis, especially in a limited-resource setting.
has issue date	2014-09-15(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2014.06.005
bibo:pmid	24925133
has license	els-covid
sha1sum (hex)	df84b7d193c3cf033fa833657b009cc4e53b7333
schema:url	https://doi.org/10.1016/j.jviromet.2014.06.005
resource representing a document's title	New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification
has PubMed Central identifier	PMC7113655
has PubMed identifier	24925133
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:df84b7d193c3cf033fa833657b009cc4e53b7333#body_text
is schema:about of	named entity 'detection limit' named entity 'endpoint' named entity 'genes' named entity 'B group' named entity 'tissue culture' named entity 'TCID50' named entity 'AMPLIFICATION' named entity 'VISUAL' named entity 'SIMPLE' named entity 'ENDPOINT' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DETECTION LIMIT' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' named entity 'LAMP' named entity '50%' named entity 'SIMULTANEOUS' named entity 'DETECTED' named entity 'SETTING' named entity 'INDICATOR' named entity 'DETECTION' named entity 'SEASONAL' named entity 'GROUP A' named entity 'CHILDREN' named entity 'GROUP' named entity 'EVALUATE' named entity 'REAL-TIME' named entity 'AMPLIFICATION' named entity 'RT-PCR' named entity 'MEDIATED' named entity 'USING' named entity 'AMPLIFY' named entity 'SWABS' named entity 'IS A' named entity 'VALIDITY' named entity 'LOOP' named entity 'REVERSE TRANSCRIPTION' named entity 'METHOD' named entity 'NEW' named entity 'DETECTION' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' named entity 'USING' named entity '1 H' named entity 'PCR POSITIVE' named entity 'DIAGNOSIS' named entity 'PATHOGEN' named entity 'RAPID' named entity 'NASOPHARYNGEAL' named entity 'TCID50' named entity 'CAUSES' named entity 'NEW' named entity 'SPECIFIC' named entity 'RESOURCE' named entity 'POSITIVE' named entity 'GENES' named entity 'PERFORMED' named entity 'PRIMERS' named entity 'METHOD' named entity 'ELDERLY' named entity 'USED' named entity 'SAMPLES' named entity 'TISSUE CULTURE' named entity 'NEGATIVE' named entity 'RESPIRATORY INFECTION' named entity 'REVERSE TRANSCRIPTASE' named entity 'LIMITED' named entity 'HRSV' named entity 'RESULTS' named entity 'RNA' named entity 'RESPIRATORY' named entity 'GROUPS' named entity 'VISUAL'

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