About: Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence

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About: Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence
Creator	Zhou, Lei Ge, Xinna Guo, Xin Yang, Hanchun Han, Jun Li, Jie Gao, Yueyi Jin, Zhonghui
Source	Medline; PMC
abstract	The recently emerged highly virulent variants of porcine epidemic and diarrhea virus (PEDV) remain a huge threat to the worldwide swine industry. Here, we describe the development of a bacterial artificial chromosome (BAC) reverse genetics system for PEDV based on two recent Chinese field isolates, namely CHM2013 and BJ2011C. Phylogenetically, CHM2013 is closely related to the vaccine strain SM98 whereas the isolate BJ2011C belongs to the GIIb group, a cluster that contains many recent pandemic strains. The full-length cDNA clones of the two isolates were constructed into BAC under the control of CMV promoter. The rescued viruses rBJ2011C and rCHM2013 were found to replicate at the kinetics similar to their respective parental viruses in cell culture. When tested in the 2-day-old pig model, rBJ2011C caused severe diarrhea of piglets with extensive damages to the intestinal epithelium, leading to 100% fatality within 48 hours. In contrast, the rCHM2013-inoculated piglets all survived with only very minor tissue damage observed. Thus, we have successfully established a convenient platform for PEDV genome manipulation. This study also represents the first description of a DNA-launched reverse genetics system for the highly virulent PEDV.
has issue date	2017-03-16(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0173998
bibo:pmid	28301551
has license	cc-by
sha1sum (hex)	e0cc48136126e2cae2d7ac3eb10a9fc786124404
schema:url	https://doi.org/10.1371/journal.pone.0173998
resource representing a document's title	Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence
has PubMed Central identifier	PMC5354467
has PubMed identifier	28301551
schema:publication	PLoS One
resource representing a document's body	covid:e0cc48136126e2cae2d7ac3eb10a9fc786124404#body_text
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