About: Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector

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About: Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector
Creator	Feng, Li Chen, Jianfei Shi, Da Shi, Hongyan Zhang, Xin Jing, Zhaoyang Liu, Jianbo Wang, Xiaobo Zhang, Jialin Han, Yuru Ji, Zhaoyang Zhang, Jiyu
source	Medline; PMC
abstract	Porcine epidemic diarrhea (PED) re-emerged in China in 2010 and is now widespread. Evidence indicates that highly virulent porcine epidemic diarrhea virus (PEDV) strains belonging to genotype G2 caused a large-scale outbreak of diarrhea. Currently, vaccines derived from PEDV classical strains do not effectively prevent infection by virulent PEDV strains, and no specific drug is available to treat the disease. RNA interference (RNAi) is a novel and effective way to cure a wide range of viruses. We constructed three short hairpin RNA (shRNA)-expressing plasmids (shR-N307, shR-N463, and shR-N1071) directed against nucleocapsid (N) and determined their antiviral activities in intestine epithelial cells infected with a classical CV777 strain and LNCT2. We verified that shR-N307, shR-N463, and shR-N1071 effectively inhibited the expression of the transfected N gene in vitro, comparable to the control shRNA. We further demonstrated the shRNAs markedly reduced PEDV CV777 and LNCT2 replication upon downregulation of N production. Therefore, this study provides a new strategy for the design of antiviral methods against coronaviruses by targeting their processivity factors.
has issue date	2019-11-02(xsd:dateTime)
bibo:doi	10.3390/vaccines7040173
bibo:pmid	31684062
has license	cc-by
sha1sum (hex)	e0e8ee783d4c465b22a44cb27e7e0f1b0d073245
schema:url	https://doi.org/10.3390/vaccines7040173
resource representing a document's title	Significant Interference with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strain Replication in Small-Intestine Epithelial Cells Using an shRNA Expression Vector
has PubMed Central identifier	PMC6963423
has PubMed identifier	31684062
schema:publication	Vaccines (Basel)
resource representing a document's body	covid:e0e8ee783d4c465b22a44cb27e7e0f1b0d073245#body_text
is schema:about of	named entity 'PRODUCTION' named entity 'DOWNREGULATION' named entity 'SPECIFIC' named entity 'SHR' named entity 'DIRECTED' named entity 'DRUG' named entity 'CURRENTLY' named entity 'CONSTRUCTED' named entity 'INFECTED' named entity 'DEMONSTRATED' named entity 'BELONGING' named entity 'RANGE' named entity 'IS A' named entity 'NUCLEOCAPSID' named entity 'TREAT' named entity 'PROVIDES' named entity 'CHINA' named entity 'RNA INTERFERENCE' named entity 'CONTROL' named entity 'STRAIN' named entity 'DO NOT' named entity 'INTERFERENCE' named entity 'SIGNIFICANT' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'genotype' named entity 'belonging' named entity 'targeting' named entity 'inhibited' named entity 'ANTIVIRAL' named entity 'VIRULENT' named entity 'HIGHLY' named entity 'IN VITRO' named entity 'EXPRESSION' named entity 'PREVENT' named entity 'REDUCED' named entity 'N GENE' named entity 'VACCINES' named entity 'TARGETING' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'WIDE' named entity 'PLASMIDS' named entity 'STUDY' named entity 'STRAINS' named entity 'EVIDENCE' named entity 'ACTIVITIES' named entity 'CLASSICAL' named entity 'REPLICATION' named entity 'EXPRESSION VECTOR' named entity 'STRAIN' named entity 'SMALL-INTESTINE' named entity 'PANDEMIC' named entity 'EPITHELIAL CELLS' named entity 'DESIGN' named entity 'SHRNA' named entity 'EPITHELIAL CELLS' named entity 'VIRUSES' named entity 'NOVEL' named entity 'REPLICATION' named entity 'LARGE' named entity 'THEIR' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'MARKEDLY' named entity 'OUTBREAK' named entity 'DETERMINED' named entity 'VERIFIED' named entity 'DERIVED' named entity 'USING' named entity 'SHRNA' named entity 'INHIBITED' named entity 'CAUSED'

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