About: Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA

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About: Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
Creator	Adachi, Koji Fujita, Motomichi Nagasawa, Michiaki
Source	Elsevier; Medline; PMC
abstract	Abstract The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for measurement of double-stranded RNA using a homogeneous time-resolved fluorescence assay. This method allowed detection of human rhinovirus (HRV), enterovirus, coxsackievirus, and murine norovirus. Furthermore, this method detected antiviral activity of a HRV 3C protease inhibitor. The assay may be useful for discovery of antiviral agents against (+) ssRNA viruses.
has issue date	2019-02-01(xsd:dateTime)
bibo:doi	10.1016/j.ab.2018.10.021
bibo:pmid	30352199
has license	els-covid
sha1sum (hex)	e1798094ce59e6068b36cbcc5648b6df624d1db6
schema:url	https://doi.org/10.1016/j.ab.2018.10.021
resource representing a document's title	Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
has PubMed Central identifier	PMC7172543
has PubMed identifier	30352199
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:e1798094ce59e6068b36cbcc5648b6df624d1db6#body_text
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