About: TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses

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About: TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses
Creator	Barr, Ian Hurt, Aeron Kelso, Anne Butler, Jeff Guarnaccia, Teagan Reading, Patrick Rockman, Steve Carolan, Louise Laurie, Karen
Source	Elsevier; Medline; PMC
abstract	Abstract The ferret is an excellent model for many human infectious diseases including influenza, SARS-CoV, henipavirus and pneumococcal infections. The ferret is also used to study cystic fibrosis and various cancers, as well as reproductive biology and physiology. However, the range of reagents available to measure the ferret immune response is very limited. To address this deficiency, high-throughput real time RT-PCR TaqMan assays were developed to measure the expression of fifteen immune mediators associated with the innate and adaptive immune responses (IFNα, IFNβ, IFNγ, IL1α, IL1β, IL2, IL4, IL6, IL8, IL10, IL12p40, IL17, Granzyme A, MCP1, TNFα), as well as four endogenous housekeeping genes (ATF4, HPRT, GAPDH, L32). These assays have been optimized to maximize reaction efficiency, reduce the amount of sample required (down to 1ng RNA per real time RT-PCR reaction) and to select the most appropriate housekeeping genes. Using these assays, the expression of each of the tested genes could be detected in ferret lymph node cells stimulated with mitogens or infected with influenza virus in vitro. These new tools will allow a more comprehensive analysis of the ferret immune responses following infection or in other disease states.
has issue date	2014-09-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2014.04.014
bibo:pmid	24797460
has license	els-covid
sha1sum (hex)	e1a0bc6deaa4e0302617cc36f048ed76db994073
schema:url	https://doi.org/10.1016/j.jviromet.2014.04.014
resource representing a document's title	TaqMan real time RT-PCR assays for detecting ferret innate and adaptive immune responses
has PubMed Central identifier	PMC7113642
has PubMed identifier	24797460
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:e1a0bc6deaa4e0302617cc36f048ed76db994073#body_text
is schema:about of	named entity 'IL2' named entity 'developed' named entity 'TNF' named entity 'infectious diseases' named entity 'immune responses' named entity 'infected' named entity 'innate' named entity 'http' named entity 'ADAPTIVE IMMUNE RESPONSES' named entity 'IN VITRO' named entity 'REACTION' named entity 'TAQMAN' named entity 'ORG' named entity 'FOLLOWING' named entity 'GENES' named entity 'THESE' named entity 'TAQMAN' named entity 'IL4' named entity 'ASSOCIATED WITH' named entity 'IL12P40' named entity 'GRANZYME A' named entity 'INFLUENZA' named entity 'NEW' named entity 'SELECT' named entity 'REQUIRED' named entity 'HAVE' named entity 'MODEL' named entity 'HOUSEKEEPING GENES' named entity 'CYSTIC FIBROSIS' named entity 'FIFTEEN' named entity 'GAPDH' named entity 'REAGENTS' named entity 'STUDY' named entity 'HUMAN' named entity 'REAL TIME RT-PCR' named entity 'ALLOW' named entity 'INFECTED' named entity 'HIGH' named entity 'RNA' named entity 'IL1' named entity 'L32' named entity 'IMMUNE MEDIATORS' named entity 'ADAPTIVE IMMUNE RESPONSES' named entity 'LICENSE' named entity 'DETECTING' named entity 'TO MEASURE' named entity 'TNF' named entity 'HPRT' named entity 'OF EACH' named entity 'INFECTION' named entity 'STIMULATED' named entity 'IFN' named entity 'USING' named entity 'NC-' named entity 'FERRET' named entity 'REAL TIME RT-PCR' named entity 'DEFICIENCY' named entity 'AVAILABLE' named entity 'REDUCE' named entity 'USED' named entity 'SARS-COV' named entity 'IL10' named entity 'PNEUMOCOCCAL INFECTIONS' named entity 'INFECTIOUS DISEASES' named entity 'IL8' named entity 'RANGE' named entity 'TOOLS' named entity 'CELLS' named entity 'COULD BE' named entity 'TESTED' named entity 'IL2' named entity 'MITOGENS' named entity 'INFLUENZA VIRUS'

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