About: Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins

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About: Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins
Creator	Anang, Saumya Kaushik, Nidhi Surjit, Milan Subramani, Chandru Tiwari, Ashutosh Kaul, Sheetal Nair, Vidya Sharma, Chandresh Ranjith-Kumar, C
Source	Medline; PMC
abstract	Hepatitis E virus (HEV) is a major cause of hepatitis in normal and organ transplant individuals. HEV open reading frame-1 encodes a polypeptide comprising of the viral nonstructural proteins as well as domains of unknown function such as the macro domain (X-domain), V, DUF3729 and Y. The macro domain proteins are ubiquitously present from prokaryotes to human and in many positive-strand RNA viruses, playing important roles in multiple cellular processes. Towards understanding the function of the HEV macro domain, we characterized its interaction partners among other HEV encoded proteins. Here, we report that the HEV X-domain directly interacts with the viral methyltransferase and the ORF3 proteins. ORF3 association with the X-domain was mediated through two independent motifs, located within its N-terminal 35aa (amino acids) and C-terminal 63-123aa. Methyltransferase interaction domain was mapped to N-terminal 30-90aa. The X-domain interacted with both ORF3 and methyltransferase through its C-terminal region, involving 66(th),67(th) isoleucine and 101(st),102(nd) leucine, conserved across HEV genotypes. Furthermore, ORF3 and methyltransferase competed with each other for associating with the X-domain. These findings provide molecular understanding of the interaction between the HEV macro domain, methyltransferase and ORF3, suggesting an important role of the macro domain in the life cycle of HEV.
has issue date	2016-04-26(xsd:dateTime)
bibo:doi	10.1038/srep25133
bibo:pmid	27113483
has license	cc-by
sha1sum (hex)	e1f84229bcacd4ea32af6127963a174f888acb09
schema:url	https://doi.org/10.1038/srep25133
resource representing a document's title	Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins
has PubMed Central identifier	PMC4844956
has PubMed identifier	27113483
schema:publication	Sci Rep
resource representing a document's body	covid:e1f84229bcacd4ea32af6127963a174f888acb09#body_text
is schema:about of	named entity 'HEPATITIS E VIRUS' named entity 'HEV' named entity 'multiple' named entity 'HEV' named entity 'interaction' named entity 'CRITICAL' named entity 'INVOLVED' named entity 'ITS' named entity 'N-TERMINAL ' named entity 'UNDERSTANDING' named entity 'ORF3' named entity 'ROLE' named entity 'DOMAIN' named entity 'ORF3' named entity 'PROVIDE' named entity 'INTERACTION' named entity 'MULTIPLE' named entity 'STRAND' named entity 'C-TERMINAL' named entity 'MEDIATED' named entity 'REGION' named entity 'LEUCINE' named entity 'REPORT' named entity '101' named entity 'MACRO' named entity 'ASSOCIATING' named entity 'HUMAN' named entity 'METHYLTRANSFERASE' named entity 'GENOTYPES' named entity 'IDENTIFICATION' named entity 'ITS' named entity 'MACRO' named entity 'PROTEINS' named entity 'INTERACTION' named entity 'OPEN' named entity 'DOMAIN' named entity 'IMPORTANT' named entity 'THESE' named entity 'CHARACTERIZED' named entity 'CELLULAR PROCESSES' named entity 'MAPPED' named entity 'RNA VIRUSES' named entity 'FINDINGS' named entity 'INVOLVING' named entity 'MOLECULAR' named entity 'PLAYING' named entity 'INDEPENDENT' named entity 'LIFE CYCLE' named entity 'MOTIFS' named entity 'ACIDS' named entity 'PARTNERS' named entity 'PRESENT' named entity 'PROKARYOTES' named entity 'ISOLEUCINE' named entity 'CONSERVED' named entity 'HEV' named entity 'METHYLTRANSFERASE' named entity 'VIRAL' named entity 'DIRECTLY' named entity 'POSITIVE' named entity 'ASSOCIATION' named entity 'FUNCTION' named entity 'ACROSS' named entity 'PROTEINS' named entity 'HERE' named entity 'VIRAL' named entity 'ENCODED' named entity 'ROLES' covid:arg/e1f84229bcacd4ea32af6127963a174f888acb09 named entity 'domain' named entity 'leucine' named entity 'independent' named entity 'encoded'

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