About: Yeast expression and characterization of SARS-CoV N protein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Yeast expression and characterization of SARS-CoV N protein
Creator	Wong, Sek-Man Cao, Shishu Luhur, Arthur Wang, Haihe
Source	Elsevier; Medline; PMC
abstract	The severe acute respiratory syndrome human coronavirus (SARS-CoV) nucleocapsid protein (N protein) is its most antigenic structural protein. The N protein gene has been cloned into a yeast expression vector pPIC9, transformed into Pichia pastoris strain GS115 and induced for expression by methanol. SDS-PAGE and Western blot showed that the N protein was expressed at a level of 3 mg/ml of culture medium. Characterization by mass spectrometry, circular dichroism and fluorescence luminescence assays showed that the expressed N protein displayed a β-sheet secondary structure in solution and it is stable in the pH range between 5.0 and 8.0. The P. pastoris-expressed N protein is believed to more closely resemble native SARS N protein than the bacterially expressed N protein.
has issue date	2005-07-18(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2005.06.010
bibo:pmid	16026862
has license	no-cc
sha1sum (hex)	e25faf74cd21615fc0254665755a19618ced6d99
schema:url	https://doi.org/10.1016/j.jviromet.2005.06.010
resource representing a document's title	Yeast expression and characterization of SARS-CoV N protein
has PubMed Central identifier	PMC7112830
has PubMed identifier	16026862
schema:publication	J Virol Methods
resource representing a document's body	covid:e25faf74cd21615fc0254665755a19618ced6d99#body_text
is schema:about of	named entity 'EXPRESSION' named entity 'Western blot' named entity 'SARS-CoV' named entity 'native' named entity 'believed' named entity 'N PROTEIN' named entity 'CHARACTERIZATION' covid:arg/e25faf74cd21615fc0254665755a19618ced6d99 named entity 'EXPRESSION' named entity 'SECONDARY STRUCTURE' named entity '90%' named entity 'CULTURE MEDIUM' named entity 'EXPRESSED' named entity 'NUCLEOCAPSID PROTEIN' named entity 'PROTEIN GENE' named entity 'CIRCULAR DICHROISM' named entity 'STRUCTURAL PROTEIN' named entity 'STRAIN' named entity 'LEVEL' named entity 'YEAST' named entity 'SARS' named entity 'ITS' named entity 'HUMAN CORONAVIRUS' named entity 'STABLE' named entity 'WESTERN BLOT' named entity 'SARS-COV' named entity 'SHEET' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'YEAST' named entity 'PICHIA PASTORIS' named entity 'INDUCED' named entity 'MG%' named entity 'MASS SPECTROMETRY' named entity 'EXPRESSION VECTOR' named entity 'NATIVE' named entity 'SDS-PAGE' named entity 'PROTEIN' named entity 'DISPLAYED' named entity 'PROTEIN ' named entity 'TRANSFORMED' named entity 'LUMINESCENCE' named entity 'PH RANGE' named entity 'CHARACTERIZATION' named entity 'SOLUTION' named entity 'N PROTEIN' named entity 'FLUORESCENCE' named entity 'METHANOL' named entity 'CLONED' named entity 'transformed' named entity 'protein' named entity 'protein' named entity 'protein' named entity 'gene' named entity 'solution' named entity 'assays' named entity 'fluorescence' named entity 'protein' named entity 'SARS' named entity 'culture medium' named entity 'circular dichroism' named entity 'SDS-PAGE' named entity 'Pichia pastoris' named entity 'yeast' named entity 'supernatant' named entity 'protein' named entity 'PMSF' named entity 'gene' named entity 'P. pastoris' named entity 'PVDF' named entity 'Western blot' named entity 'nucleocapsid' named entity 'excitation wavelength' named entity 'SDS-PAGE' named entity 'metal ions' named entity '2.0' named entity 'P. pastoris' named entity 'milk' named entity 'post-translationally modified'

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