About: Expression and purification of SARS coronavirus proteins using SUMO-fusions

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About: Expression and purification of SARS coronavirus proteins using SUMO-fusions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Expression and purification of SARS coronavirus proteins using SUMO-fusions
Creator	Weiss, Susan Navas-Martin, Sonia Kazi, Lubna Lim, Peter Zuo, Xun Hall, John Li, Shuisen Shoo, Joshua Tan, Robin Tran, Hiep Mattern, Michael Butt, Tauseef Sterner, David Sarawanos, Stefan
source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome coronavirus (SARS-CoV) proteins belong to a large group of proteins that is difficult to express in traditional expression systems. The ability to express and purify SARS-CoV proteins in large quantities is critical for basic research and for development of pharmaceutical agents. The work reported here demonstrates: (1) fusion of SUMO (small ubiquitin-related modifier), a 100 amino acid polypeptide, to the N-termini of SARS-CoV proteins dramatically enhances expression in Escherichia coli cells and (2) 6× His-tagged SUMO-fusions facilitate rapid purification of the viral proteins on a large scale. We have exploited the natural chaperoning properties of SUMO to develop an expression system suitable for proteins that cannot be expressed by traditional methodologies. A unique feature of the system is the SUMO tag, which enhances expression, facilitates purification, and can be efficiently cleaved by a SUMO-specific protease to generate native protein with a desired N-terminus. We have purified various SARS-CoV proteins under either native or denaturing conditions. These purified proteins have been used to generate highly specific polyclonal antibodies. Our study suggests that the SUMO-fusion technology will be useful for enhancing expression and purification of the viral proteins for structural and functional studies as well as for therapeutic uses.
has issue date	2005-07-31(xsd:dateTime)
bibo:doi	10.1016/j.pep.2005.02.004
bibo:pmid	15939295
has license	els-covid
sha1sum (hex)	e27bfcbf7f7a6c0d0e30aa945af857c240f6cd6b
schema:url	https://doi.org/10.1016/j.pep.2005.02.004
resource representing a document's title	Expression and purification of SARS coronavirus proteins using SUMO-fusions
has PubMed Central identifier	PMC7129641
has PubMed identifier	15939295
schema:publication	Protein Expression and Purification
resource representing a document's body	covid:e27bfcbf7f7a6c0d0e30aa945af857c240f6cd6b#body_text
is schema:about of	named entity 'proteins' named entity 'enhancing' named entity 'develop' named entity 'studies' named entity 'These' named entity 'Escherichia coli' named entity 'expression system' named entity 'cleaved' named entity 'NATIVE PROTEIN' named entity 'SARS CORONAVIRUS' named entity 'VARIOUS' named entity 'DENATURING' named entity 'EXPRESSION' named entity 'SUITABLE' named entity 'SARS-COV' named entity 'THERAPEUTIC USES' named entity 'ESCHERICHIA COLI' named entity '282' named entity 'PHARMACEUTICAL' named entity 'HIS-' named entity 'REPORTED' named entity 'HIGHLY' named entity 'TAGGED' named entity 'PURI' named entity 'STUDIES' named entity 'CRITICAL' named entity 'HAVE' named entity 'METHODOLOGIES' named entity 'HERE' named entity 'AMINO ACID' named entity 'USED' named entity 'POLYCLONAL ANTIBODIES' named entity 'RAPID' named entity 'SUMO TAG' named entity 'EXPRESSED' named entity 'SYSTEM' named entity 'VIRAL PROTEINS' named entity 'SYSTEMS' named entity 'N-TERMINUS' named entity 'PROTEIN ' named entity 'CELLS' named entity 'THESE' named entity 'EITHER' named entity 'PROTEINS' named entity 'USING' named entity 'LARGE SCALE' named entity 'PURIFY' named entity 'EXPRESSION SYSTEM' named entity 'DEVELOPMENT' named entity 'UNIQUE' named entity 'SUMO' named entity 'QUANTITIES' named entity 'EXPRESSION' named entity 'FUSIONS' named entity 'TO EXPRESS' named entity 'WORK' named entity 'USEFUL' named entity 'PROTEINS' named entity 'LARGE' named entity 'ENHANCING' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'OUR' named entity 'FUNCTIONAL' named entity 'CLEAVED' named entity 'TRADITIONAL' named entity 'STRUCTURAL' named entity 'PROTEASE' named entity 'POLYPEPTIDE'

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