About: The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis

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About: The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis
Creator	Liao, Ming Xu, Siqi Zhang, Bin Chen, Aihua Feng, Saixiang Pan, Zhichao Wang, Xiaobing Yu, Huiwen
Source	PMC
abstract	Lipooligosaccharides (LOSs) are virulence determinants of Glaesserella parasuis, a pathogen of the respiratory tract of pigs. We previously reported that disruption of the galU or galE gene in G. parasuis results in increased sensitivity to porcine serum, indicating that the galactose catabolism pathway is required for polysaccharide formation in G. parasuis. Here, we evaluated the role of the HAPS_0849 gene in LOS synthesis. The G. parasuis SC096 HAPS_0849 mutant produced a highly truncated LOS molecule, although a small fraction of intact LOS was still observed, and this mutant was found to be more sensitive to serum than the parental strain. HAPS_0849 was overexpressed and purified for biochemical assays, and this protein exhibited phosphoglucomutase (PGM) activity. Heterologous expression of a pgm gene from Escherichia coli in the HAPS_0849 mutant led to restoration of the wild-type LOS glycoform, further demonstrating the PGM function of HAPS_0849 in G. parasuis. The autoagglutination and biofilm formation ability of this strain were also investigated. Disruption of HAPS_0849 led to an increased tendency to autoagglutinate and form more biofilms, and these enhanced phenotypes were observed in the absence of glucose. In addition, LOSs from HAPS_0849, galU and lgtB mutants had similar truncated glycoforms, while LOSs from the galE and lex-1 mutants exhibited another type of defective LOS pattern. These findings imply that HAPS_0849 may function upstream of GalU in the generation of glucose 1-phosphate. In conclusion, our results preliminarily described the functions of HAPS_0849 in G. parasuis, and this gene was partially required for LOS synthesis.
has issue date	2020-07-31(xsd:dateTime)
bibo:doi	10.1186/s13567-020-00822-9
has license	cc-by
sha1sum (hex)	e398ca4ccea27c4d0d4d0264b153ecdfcff866e2
schema:url	https://doi.org/10.1186/s13567-020-00822-9
resource representing a document's title	The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis
has PubMed Central identifier	PMC7393335
schema:publication	Vet Res
resource representing a document's body	covid:e398ca4ccea27c4d0d4d0264b153ecdfcff866e2#body_text
is schema:about of	named entity 'HAPS' named entity 'glucose' named entity 'expression' named entity 'strain' named entity 'galU' named entity 'pathogen' named entity 'led' named entity 'HAPS' named entity 'phosphoglucomutase' named entity 'BIOCHEMICAL' named entity 'PGM' named entity 'FUNCTION' named entity 'HETEROLOGOUS EXPRESSION' named entity 'REQUIRED' named entity 'HAPS' named entity 'serum' named entity 'HAPS' named entity 'protein' named entity 'HAPS' named entity 'polysaccharide' named entity 'ability' named entity 'LOS' named entity 'glucose 1-phosphate' named entity 'purified' named entity 'demonstrating' named entity 'virulence determinants' named entity 'glucose' named entity 'mutants' named entity 'glycoform' named entity 'serum' named entity 'serum' named entity 'biofilms' named entity 'biofilm formation' named entity 'gene' named entity 'porcine' named entity 'gene' named entity 'protein' named entity 'lgtB' named entity 'phenotypes' named entity 'lipooligosaccharide' named entity 'biochemical assays' named entity 'serum' named entity 'TSA' named entity 'UDP-galactose' named entity 'glycoform' named entity 'substrate' named entity 'glucose' named entity 'lgtB' named entity 'glucose' named entity 'wild-type' named entity 'galactose' named entity 'galactose' named entity 'polysaccharides' named entity 'G1P' named entity 'polysaccharides' named entity 'standard deviations' named entity 'serum' named entity 'Phosphoglucomutase' named entity 'gene' named entity 'substrate' named entity 'glucose' named entity 'bacterial colonies' named entity 'trypticase soy broth' named entity 'gene' named entity 'PCR' named entity 'uridine diphosphate glucose' named entity 'ultrasonication' named entity 'bacteria' named entity 'swine' named entity 'biosynthesis' named entity 'endogenous' named entity 'mannose' named entity 'primers' named entity 'molecule' named entity 'substrate' named entity 'glucose'

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