About: Precise large deletions by the PCR-based overlap extension method

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Precise large deletions by the PCR-based overlap extension method
Creator	Brian, David Senanayake, Savithra
Source	PMC
abstract	The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
has issue date	1995-01-01(xsd:dateTime)
bibo:doi	10.1007/bf02907467
bibo:pmid	8521036
has license	no-cc
sha1sum (hex)	e3c78c86ca2843975e37a2f5c36fb0665ae94f7b
schema:url	https://doi.org/10.1007/bf02907467
resource representing a document's title	Precise large deletions by the PCR-based overlap extension method
has PubMed Central identifier	PMC7090436
has PubMed identifier	8521036
schema:publication	Mol Biotechnol
resource representing a document's body	covid:e3c78c86ca2843975e37a2f5c36fb0665ae94f7b#body_text
is schema:about of	named entity 'LARGE' named entity 'conventional' named entity 'USING' named entity 'AUTHORS' named entity 'LENGTH' named entity 'BASED' named entity 'PCR' named entity 'PREPARATION' named entity 'TIME' named entity 'CONSUMING' named entity 'EXTENSION' named entity 'BASED' named entity 'EXTENSION' named entity 'MUTAGENESIS' named entity 'EFFICIENT' named entity 'NTS' named entity 'OVERLAP' named entity 'LARGE' named entity 'PRECISE' named entity 'TECHNIQUES' named entity 'TEMPLATE' named entity 'PRECISE' named entity 'LOOP' named entity 'SIMPLE' named entity 'CONVENTIONAL' named entity 'METHOD' named entity 'SINGLE-STRANDED DNA' named entity 'GENE SPLICING' named entity 'DESCRIBE' named entity 'PCR' named entity 'METHOD' named entity 'OVERLAP' named entity 'GENERATING' covid:arg/e3c78c86ca2843975e37a2f5c36fb0665ae94f7b named entity 'time' named entity 'length' named entity 'precise' named entity 'large' named entity 'deletions' named entity 'Deletions' named entity 'Extension Method' named entity 'ethanol' named entity 'PCR' named entity 'gene' named entity 'ORF2' named entity 'cloned' named entity 'Taq DNA polymerase' named entity 'primers' named entity 'phenol-chloroform' named entity 'in-frame' named entity 'electrophoretically' named entity 'Department of Microbiology' named entity 'bovine coronavirus' named entity 'oligonucleotide primers' named entity 'PCR' named entity 'molecule' named entity 'PCR' named entity 'ORFs' named entity 'cloned' named entity 'restriction endonuclease' named entity 'mutagenesis' named entity 'in-frame' named entity '2, 3' named entity 'agarose gel electrophoresis' named entity 'vectors' named entity 'cloned' named entity 'gene splicing' named entity 'clone' named entity 'open reading frame' named entity 'Promega' named entity 'in-frame' named entity 'ssDNA' named entity 'subgenomic' named entity 'RNA' named entity 'PCR-based' named entity 'mineral oil' named entity 'homologous' named entity 'PCR' named entity 'University of Tennessee' named entity 'genome'

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