About: Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference

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About: Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference
Creator	Shi, Yi Cai, You-Min Cheng, Guo-Feng Fu, Zhi-Qiang Jin, Ya-Mei Jin, You-Xin Lin, Jiao-Jiao Zhou, Yuan-Cong
source	Medline; PMC
abstract	Abstract The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription‐polymerase chain reaction (RT‐PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose‐dependent. RT‐PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied. Edited by: Wang‐Yi LIU
has issue date	2005-06-17(xsd:dateTime)
bibo:doi	10.1111/j.1745-7270.2005.00058.x
bibo:pmid	15944753
has license	no-cc
sha1sum (hex)	e544b99da0887ce13959491d46b793149c96c60b
schema:url	https://doi.org/10.1111/j.1745-7270.2005.00058.x
resource representing a document's title	Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference
has PubMed Central identifier	PMC7185640
has PubMed identifier	15944753
schema:publication	Acta Biochim Biophys Sin (Shanghai)
resource representing a document's body	covid:e544b99da0887ce13959491d46b793149c96c60b#body_text
is schema:about of	named entity 'dsRNA' named entity 'immunofluorescence' named entity 'POLYMERASE CHAIN REACTION' named entity 'DOSE-DEPENDENT' named entity 'PAIRING' named entity 'PROTEIN GENE' named entity 'REVEALED' named entity 'SCHISTOSOMA JAPONICUM' named entity 'PCR ANALYSIS' named entity 'RESULTS' named entity 'LEVEL' named entity 'FEMALE' named entity 'INHIBITION' covid:arg/e544b99da0887ce13959491d46b793149c96c60b named entity 'male' named entity 'pairing' named entity 'transcript' named entity 'dsRNA' named entity 'worms' named entity 'protein' named entity 'siRNA' named entity 'parasites' named entity 'worm' named entity 'Schistosoma japonicum' named entity 'RT-PCR' named entity 'Schistosoma japonicum' named entity 'Shanghai' named entity 'RT-PCR' named entity 'Trizol' named entity 'schistosomes' named entity 'Lactalbumin' named entity 'fluorescence' named entity 'aseptically' named entity 'gene expression' named entity 'gene' named entity 'Shanghai Institute of Biochemistry and Cell Biology' named entity 'RT-PCR' named entity 'gene' named entity 'hydrocortisone' named entity 'sexually immature' named entity 'infection' named entity 'dsRNA' named entity 'RNAi' named entity 'parasites' named entity 'parasites' named entity 'RNA interference' named entity 'serum' named entity 'parasites' named entity 'schistosome' named entity 'critical role' named entity 'dose-dependent' named entity 'Schistosoma japonicum' named entity 'parasites' named entity 'Parasites' named entity 'hydrolysate' named entity 'Shanghai' named entity 'Fluorescence' named entity 'immunofluorescence' named entity 'protein' named entity 'insulin' named entity 'granuloma' named entity 'parasites' named entity 'dsRNA' named entity 'dsRNA' named entity 'dsRNA' named entity 'parasites' named entity 'sequence similarity' named entity 'dsRNA' named entity 'Shanghai' named entity 'protein' named entity 'gene' named entity 'Schistosomiasis' named entity '1:300' named entity 'dsRNA'

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