Facets (new session)
Description
Metadata
Settings
owl:sameAs
Inference Rule:
b3s
b3sifp
dbprdf-label
facets
http://dbpedia.org/resource/inference/rules/dbpedia#
http://dbpedia.org/resource/inference/rules/opencyc#
http://dbpedia.org/resource/inference/rules/umbel#
http://dbpedia.org/resource/inference/rules/yago#
http://dbpedia.org/schema/property_rules#
http://www.ontologyportal.org/inference/rules/SUMO#
http://www.ontologyportal.org/inference/rules/WordNet#
http://www.w3.org/2002/07/owl#
ldp
oplweb
skos-trans
virtrdf-label
None
About:
Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression
Goto
Sponge
NotDistinct
Permalink
An Entity of Type :
schema:ScholarlyArticle
, within Data Space :
wasabi.inria.fr
associated with source
document(s)
Type:
Academic Article
research paper
schema:ScholarlyArticle
New Facet based on Instances of this Class
Attributes
Values
type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression
Creator
Chen, Chi-Min
Chiou, Ming-Tang
Chen, Chuan-Mu
Chuang, Chin-Kai
Hsiao, Kai-Hsuan
Hung, Shao-Wen
Peng, Su-Hei
Su, Yu-Hsiu
Tu, Ching-Fu
Yang, Tien-Shuh
Yen, Chon-Ho
Chen,
Taipei,
Kun, Chao
Ltd, Biotech
Taiwan, R
Source
Medline; PMC
abstract
The porcine epidemic diarrhoea virus (PEDV) devastates the health of piglets but may not infect piglets whose CMP-N-glycolylneuraminic acid hydroxylase (CMAH) gene is mutated (knockouts, KO) by using CRISPR/Cas9 gene editing techniques. This hypothesis was tested by using KO piglets that were challenged with PEDV. Two single-guide RNAs targeting the CMAH gene and Cas9 mRNA were microinjected into the cytoplasm of newly fertilized eggs. Four live founders generated and proven to be biallelic KO, lacking detectable N-glycolylneuraminic acid (NGNA). The founders were bred, and homozygous offspring were obtained. Two-day-old (in exps. I, n = 6, and III, n = 15) and 3-day-old (in exp. II, n = 9) KO and wild-type (WT, same ages in respective exps.) piglets were inoculated with TCID(50) 1x10(3) PEDV and then fed 20 mL of infant formula (in exps. I and II) or sow’s colostrum (in exp. III) every 4 hours. In exp. III, the colostrum was offered 6 times and was then replaced with Ringer/5% glucose solution. At 72 hours post-PEDV inoculation (hpi), the animals either deceased or euthanized were necropsied and intestines were sampled. In all 3 experiments, the piglets showed apparent outward clinical manifestations suggesting that infection occurred despite the CMAH KO. In exp. I, all 6 WT piglets and only 1 of 6 KO piglets died at 72 hpi. Histopathology and immunofluorescence staining showed that the villus epithelial cells of WT piglets were severely exfoliated, but only moderate exfoliation and enterocyte vacuolization was observed in KO piglets. In exp. II, delayed clinical symptoms appeared, yet the immunofluorescence staining/histopathologic inspection (I/H) scores of the two groups differed little. In exp. III, the animals exhibited clinical and pathological signs after inoculation similar to those in exp. II. These results suggest that porcine CMAH KO with nullified NGNA expression are not immune to PEDV but that this KO may lessen the severity of the infection and delay its occurrence.
has issue date
2019-05-29
(
xsd:dateTime
)
bibo:doi
10.1371/journal.pone.0217236
bibo:pmid
31141512
has license
cc-by
sha1sum (hex)
e67d66fb0773322520aa527d533c6cffb08016a2
schema:url
https://doi.org/10.1371/journal.pone.0217236
resource representing a document's title
Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression
has PubMed Central identifier
PMC6541307
has PubMed identifier
31141512
schema:publication
PLoS One
resource representing a document's body
covid:e67d66fb0773322520aa527d533c6cffb08016a2#body_text
is
schema:about
of
named entity 'cytoplasm'
named entity 'infection'
named entity 'observed'
named entity 'animals'
named entity 'mutated'
named entity 'piglets'
named entity 'pathological'
named entity 'doi'
named entity 'susceptibility'
named entity 'gene'
named entity 'ACID'
covid:arg/e67d66fb0773322520aa527d533c6cffb08016a2
named entity 'VACUOLIZATION'
named entity 'IMMUNOFLUORESCENCE STAINING'
named entity '28I'
named entity 'MRNA'
named entity 'CMP-N-GLYCOLYLNEURAMINIC ACID'
named entity 'GENE'
named entity 'HPI'
named entity 'INSPECTION'
named entity 'HYPOTHESIS'
named entity '72 HOURS'
named entity 'CFT'
named entity 'SALARIES'
named entity 'CLINICAL SYMPTOMS'
named entity 'BUT'
named entity 'SOW'
named entity 'NULLIFIED'
named entity 'PROVIDED'
named entity 'NGNA'
named entity 'SAMPLED'
named entity 'RNAS'
named entity 'OFFERED'
named entity 'COLOSTRUM'
named entity 'FED'
named entity 'RESULTS'
named entity 'TECHNIQUES'
named entity 'HISTOPATHOLOGY'
named entity 'PATHOLOGICAL'
named entity 'ITS'
named entity 'DELAY'
named entity 'HEALTH'
named entity 'REPLACED'
named entity 'INFANT FORMULA'
named entity 'INTESTINES'
named entity 'ROC'
named entity 'EGGS'
named entity 'DIED'
named entity 'CLINICAL'
named entity 'ACID'
named entity 'LIVE'
named entity 'BRED'
named entity 'OCCURRENCE'
named entity 'TARGETING'
named entity 'CAS9'
named entity '27S'
named entity 'SEVERITY'
named entity 'OCCURRED'
named entity 'DETECTABLE'
named entity 'SEVERELY'
named entity 'LITTLE'
named entity 'SCORES'
named entity 'AGES'
named entity 'MODERATE'
named entity 'POST'
named entity 'TO SUPPORT'
◂◂ First
◂ Prev
Next ▸
Last ▸▸
Page 1 of 11
Go
Faceted Search & Find service v1.13.91 as of Mar 24 2020
Alternative Linked Data Documents:
Sponger
|
ODE
Content Formats:
RDF
ODATA
Microdata
About
OpenLink Virtuoso
version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software