About: JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells

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About: JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells
Creator	Mizutani, Tetsuya Fukushi, Shuetsu Morikawa, Shigeru Saijo, Masayuki Kurane, Ichiro
Source	Elsevier; Medline; PMC
abstract	Abstract Persistence was established after most of the SARS-CoV-infected Vero E6 cells died. RNA of the defective interfering virus was not observed in the persistently infected cells by Northern blot analysis. SARS-CoV diluted to 2 PFU failed to establish persistence, suggesting that some particular viruses in the seed virus did not induce persistent infection. Interestingly, a viral receptor, angiotensin converting enzyme (ACE)-2, was down-regulated in persistently infected cells. G418-selected clones established from parent Vero E6 cells, which were transfected with a plasmid containing the neomycin resistance gene, were infected with SARS-CoV, resulting in a potential cell population capable of persistence in Vero E6 cells. Our previous studies demonstrated that signaling pathways of extracellular signal-related kinase (ERK1/2), c-Jun N-terminal protein kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3′-kinase (PI3K)/Akt were activated in SARS-CoV-infected Vero E6 cells. Previous studies also showed that the activation of p38 MAPK by viral infection-induced apoptosis, and a weak activation of Akt was not sufficient to protect from apoptosis. In the present study, we showed that the inhibitors of JNK and PI3K/Akt inhibited the establishment of persistence, but those of MAPK/ERK kinase (MEK; as an inhibitor for ERK1/2) and p38 MAPK did not. These results indicated that two signaling pathways of JNK and PI3K/Akt were important for the establishment of persistence in Vero E6 cells.
has issue date	2005-06-30(xsd:dateTime)
bibo:doi	10.1016/j.bbadis.2005.04.004
bibo:pmid	15916886
has license	els-covid
sha1sum (hex)	e6f498d287af817fe9cf8dca33bb01163db27642
schema:url	https://doi.org/10.1016/j.bbadis.2005.04.004
resource representing a document's title	JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells
has PubMed Central identifier	PMC7125767
has PubMed identifier	15916886
schema:publication	Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
resource representing a document's body	covid:e6f498d287af817fe9cf8dca33bb01163db27642#body_text
is schema:about of	named entity 'cell' named entity 'cells' named entity 'inhibitors' named entity 'potential' named entity 'studies' named entity 'cells' named entity 'PERSISTENT' named entity 'CELL POPULATION' named entity 'ERK1' named entity 'RNA' named entity 'RESULTS' named entity 'INFECTED' named entity 'CELLS' named entity 'NOT OBSERVED' named entity 'DILUTED' named entity 'PERSISTENCE' named entity 'PLASMID' named entity 'ESTABLISHMENT' named entity 'PFU' named entity 'STUDY' named entity 'INDICATED' named entity 'CAPABLE' named entity 'PERSISTENT INFECTION' named entity 'MOST OF' named entity 'PI3K' named entity 'DEMONSTRATED' named entity 'SIGNAL' named entity 'FAILED' named entity 'WEAK' named entity 'EXTRACELLULAR' named entity 'ACTIVATED' named entity 'ACTIVATION OF P38 MAPK' named entity 'C-JUN' named entity 'DEFECTIVE' covid:arg/e6f498d287af817fe9cf8dca33bb01163db27642 named entity 'CELLS' named entity 'PI3K' named entity 'IMPORTANT' named entity 'SEED' named entity 'RAPID' named entity 'N-TERMINAL ' named entity 'JNK' named entity 'DIED' named entity 'STUDIES' named entity 'KINASE' named entity 'PHOSPHATIDYLINOSITOL' named entity 'SIGNALING PATHWAYS' named entity 'SARS-COV' named entity 'P38 MITOGEN-ACTIVATED PROTEIN KINASE' named entity 'RELATED' named entity 'BUT' named entity 'VIRUS' named entity 'INTERFERING VIRUS' named entity 'CLONES' named entity 'VIRAL' named entity 'ESTABLISHED' named entity 'PARENT' named entity 'INHIBITOR' named entity 'SUFFICIENT' named entity 'SARS-COV INFECTION' named entity 'INDUCE' named entity 'DID' named entity 'REPORT' named entity 'REQUIRED' named entity 'SIGNALING PATHWAYS' named entity 'ESTABLISH' named entity 'ACTIVATION' named entity 'MAPK' named entity 'PREVIOUS' named entity 'VERO' named entity 'OUR' named entity 'JNK' named entity 'ESTABLISHING' named entity 'VERO' named entity 'PARTICULAR' named entity 'DOWN-REGULATED' named entity 'AKT'

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