About: Multiple Cis-acting elements modulate programmed -1 ribosomal frameshifting in Pea enation mosaic virus

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About: Multiple Cis-acting elements modulate programmed -1 ribosomal frameshifting in Pea enation mosaic virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multiple Cis-acting elements modulate programmed -1 ribosomal frameshifting in Pea enation mosaic virus
Creator	Gao, Feng Simon, Anne
source	Medline; PMC
abstract	Programmed -1 ribosomal frameshifting (-1 PRF) is used by many positive-strand RNA viruses for translation of required products. Despite extensive studies, it remains unresolved how cis-elements just downstream of the recoding site promote a precise level of frameshifting. The Umbravirus Pea enation mosaic virus RNA2 expresses its RNA polymerase by -1 PRF of the 5′-proximal ORF (p33). Three hairpins located in the vicinity of the recoding site are phylogenetically conserved among Umbraviruses. The central Recoding Stimulatory Element (RSE), located downstream of the p33 termination codon, is a large hairpin with two asymmetric internal loops. Mutational analyses revealed that sequences throughout the RSE and the RSE lower stem (LS) structure are important for frameshifting. SHAPE probing of mutants indicated the presence of higher order structure, and sequences in the LS may also adapt an alternative conformation. Long-distance pairing between the RSE and a 3′ terminal hairpin was less critical when the LS structure was stabilized. A basal level of frameshifting occurring in the absence of the RSE increases to 72% of wild-type when a hairpin upstream of the slippery site is also deleted. These results suggest that suppression of frameshifting may be needed in the absence of an active RSE conformation.
has issue date	2016-01-29(xsd:dateTime)
bibo:doi	10.1093/nar/gkv1241
bibo:pmid	26578603
has license	cc-by
sha1sum (hex)	e745a55d651d2f45443c39ed949185d8f968500a
schema:url	https://doi.org/10.1093/nar/gkv1241
resource representing a document's title	Multiple Cis-acting elements modulate programmed -1 ribosomal frameshifting in Pea enation mosaic virus
has PubMed Central identifier	PMC4737148
has PubMed identifier	26578603
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:e745a55d651d2f45443c39ed949185d8f968500a#body_text
is schema:about of	named entity 'central' named entity 'products' named entity 'Long-distance' named entity 'pairing' named entity 'wild-type' »more»

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