About: Development of a novel detection system for microbes from bovine diarrhea by real-time PCR

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About: Development of a novel detection system for microbes from bovine diarrhea by real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a novel detection system for microbes from bovine diarrhea by real-time PCR
Creator	Katayama, Yukie Masuda, Tsuneyuki Mizutani, Tetsuya Nagai, Makoto Oba, Mami Omatsu, Tsutomu Tsuchiaka, Shinobu Furuya, Tetsuya Sugimura, Satoshi Kanda, Shuhei Kobayashi, Suguru Komatsu, Natsumi Yokoyama, Tadashi
Source	Medline; PMC
abstract	Diarrhea in cattle is one of the most economically costly disorders, decreasing milk production and weight gain. In the present study, we established a novel simultaneous detection system using TaqMan real-time PCR designed as a system for detection of microbes from bovine diarrhea using real-time PCR (referred to as Dembo-PCR). Dembo-PCR simultaneously detects a total of 19 diarrhea-causing pathogens, including viruses, bacteria and protozoa. Specific primer–probe sets were newly designed for 7 pathogens and were synthesized on the basis of previous reports for 12 pathogens. Assays were optimized to react under the same reaction conditions. The PCR efficiency and correlation coefficient (R(2)) of standard curves for each assay were more than 80% and 0.9766, respectively. Furthermore, the sensitivity of Dembo-PCR in fecal sample analysis was measured with feces spiked with target pathogens or synthesized DNA that included specific nucleotide target regions. The resulting limits of detection (LOD) for virus-spiked samples, bacteria and DNA fragments were 0.16–1.6 TCID(50) (PFU/reaction), 1.3–13 CFU/reaction and 10–100 copies/reaction, respectively. All reactions showed high sensitivity in pathogen detection. A total of 8 fecal samples, collected from 6 diarrheic cattle, 1 diarrheic calf and 1 healthy cow, were tested using Dembo-PCR to validate the assay’s clinical performance. The results revealed that bovine coronavirus had infected all diarrheic adult cattle and that bovine torovirus had infected the diarrheic calf. These results suggest that Dembo-PCR may be a powerful tool for diagnosing infectious agents in cattle diarrhea.
has issue date	2015-11-30(xsd:dateTime)
bibo:doi	10.1292/jvms.15-0552
bibo:pmid	26616156
has license	cc-by-nc-nd
sha1sum (hex)	e7b8761e03a3ce7edd5c0df23946c4ac8618c708
schema:url	https://doi.org/10.1292/jvms.15-0552
resource representing a document's title	Development of a novel detection system for microbes from bovine diarrhea by real-time PCR
has PubMed Central identifier	PMC4829504
has PubMed identifier	26616156
schema:publication	J Vet Med Sci
resource representing a document's body	covid:e7b8761e03a3ce7edd5c0df23946c4ac8618c708#body_text
is schema:about of	named entity 'basis' named entity 'collected' named entity 'optimized' named entity 'torovirus' named entity 'microbes' named entity 'diarrhea' named entity 'bovine' named entity 'BOVINE' covid:arg/e7b8761e03a3ce7edd5c0df23946c4ac8618c708 named entity 'DETECTION' named entity 'SYSTEM' named entity 'NOVEL' named entity 'MORE THAN' named entity 'COPIES' named entity 'STANDARD' named entity 'PCR' named entity 'PATHOGEN DETECTION' named entity 'RESULTS' named entity 'COLLECTED' named entity 'EFFICIENCY' named entity 'BACTERIA ' named entity 'RESULTING' named entity 'CAUSING' named entity 'SYSTEM' named entity 'INFECTIOUS AGENTS' named entity 'ADULT' named entity 'PATHOGENS' named entity 'BOVINE CORONAVIRUS' named entity '100' named entity 'SUGGEST' named entity 'THESE' named entity 'TAQMAN' named entity '27S' named entity 'HIGH SENSITIVITY' named entity 'REACTION' named entity 'REVEALED' named entity 'SENSITIVITY' named entity 'LIMITS OF DETECTION' named entity 'NUCLEOTIDE' named entity 'ESTABLISHED' named entity 'SIMULTANEOUS' named entity 'DEVELOPMENT' named entity 'DIARRHEA' named entity 'MICROBES' named entity 'STUDY' named entity 'BOVINE' named entity 'TOTAL' named entity 'TOOL' named entity 'MICROBES' named entity 'MAY BE A' named entity 'SAMPLES' named entity 'ASSAY' named entity 'BOVINE TOROVIRUS' named entity 'INCLUDING' named entity 'CALF' named entity '1.6' named entity 'CORRELATION COEFFICIENT' named entity 'NEWLY' named entity 'POWERFUL' named entity '0.16' named entity 'VIRUS' named entity 'CATTLE' named entity 'DIARRHEA' named entity 'BASIS' named entity 'SAMPLE ANALYSIS' named entity 'CONDITIONS' named entity 'VIRUSES' named entity 'DNA' named entity 'MILK PRODUCTION'

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