About: Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann

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About: Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann
Creator	Shen, Jie Liu, Zhenguo Chen, Guohong Corona, Miguel Ji, Ting Liang, Qin Shen, Fang Wu, Liming
Source	Medline; PMC
abstract	BACKGROUND: Most of the proteins contained in royal jelly (RJ) are secreted from the hypopharyngeal glands (HG) of young bees. Although generic protein composition of RJ has been investigated, little is known about how age-dependent changes on HG secretion affect RJ composition and their biological consequences. In this study, we identified differentially expressed proteins (DEPs) during HG development by using the isobaric tag for relative and absolute quantification (iTRAQ) labeling technique. This proteomic method increases the potential for new protein discovery by improving the identification of low quantity proteins. RESULTS: A total of 1282 proteins were identified from five age groups of worker bees, 284 of which were differentially expressed. 43 (15.1%) of the DEPs were identified for the first time. Comparison of samples at day 6, 9, 12, and 16 of development relative to day 3 led to the unambiguous identification of 112, 117, 127, and 127 DEPs, respectively. The majority of these DEPs were up-regulated in the older worker groups, indicating a substantial change in the pattern of proteins expressed after 3 days. DEPs were identified among all the age groups, suggesting that changes in protein expression during HG ontogeny are concomitant with different states of worker development. A total of 649 proteins were mapped to canonical signaling pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG), which were preferentially associated with metabolism and biosynthesis of secondary metabolites. More than 10 key high-abundance proteins were involved in signaling pathways related to ribosome function and protein processing in the endoplasmic reticulum. The results were validated by qPCR. CONCLUSION: Our approach demonstrates that HG experienced important changes in protein expression during its ontogenic development, which supports the secretion of proteins involved in diverse functions in adult workers beyond its traditional role in royal jelly production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-665) contains supplementary material, which is available to authorized users.
has issue date	2014-08-08(xsd:dateTime)
bibo:doi	10.1186/1471-2164-15-665
bibo:pmid	25103401
has license	cc-by
sha1sum (hex)	e840685d875ae1d0d6c08a0176f2151b2765a0d0
schema:url	https://doi.org/10.1186/1471-2164-15-665
resource representing a document's title	Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann
has PubMed Central identifier	PMC4141115
has PubMed identifier	25103401
schema:publication	BMC Genomics
resource representing a document's body	covid:e840685d875ae1d0d6c08a0176f2151b2765a0d0#body_text
is schema:about of	named entity 'This' named entity 'Encyclopedia' named entity 'protein' named entity 'quantification' named entity 'total' named entity 'Results' named entity 'Apis mellifera carnica' named entity 'honeybee' covid:arg/e840685d875ae1d0d6c08a0176f2151b2765a0d0 named entity 'UP-REGULATED' named entity 'QPCR' named entity 'QUANTITY' named entity 'YOUNG' named entity 'STUDY' named entity 'ENDOPLASMIC RETICULUM' named entity 'AGE' named entity 'COMPARISON' named entity 'RELATIVE' named entity 'PROTEIN ' named entity 'PROTEOMIC' named entity 'GENERIC' named entity 'CONTAINED IN' named entity 'THEIR' named entity 'ASSOCIATED WITH' named entity 'WORKER' named entity 'INDICATING' named entity 'GROUPS' named entity 'DAY 6' named entity 'LITTLE' named entity 'NEW' named entity 'ABSOLUTE' named entity 'COMPOSITION' named entity 'SIGNALING PATHWAYS' named entity 'STATES' named entity 'PROTEIN EXPRESSION' named entity 'METHOD' named entity 'LABELING TECHNIQUE' named entity 'IDENTIFICATION' named entity 'THESE' named entity 'RELATIVE TO' named entity 'CONCOMITANT' named entity 'KEY' named entity 'HOW' named entity 'KYOTO ENCYCLOPEDIA OF GENES AND GENOMES' named entity '112' named entity 'MOST OF' named entity 'FUNCTION' named entity 'PATTERN' named entity 'DEVELOPMENT' named entity 'TAG' named entity 'RIBOSOME' named entity 'SAMPLES' named entity 'AFFECT' named entity 'BEES' named entity 'CHANGE IN' named entity 'ONTOGENY' named entity 'LOW' named entity 'IDENTIFIED' named entity 'EXPRESSED' named entity 'POTENTIAL' named entity 'DEPS' named entity 'ABOUT' named entity 'SECRETED' named entity 'PROTEOMICS ANALYSIS' named entity 'KNOWN' named entity 'CHANGES' named entity 'IMPROVING' named entity 'DAYS' named entity 'ACTIVITY' named entity 'PROTEINS' named entity 'VALIDATED' named entity 'HONEYBEE' named entity 'PROTEIN EXPRESSION' named entity 'RESULTS'

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