About: Human alveolar epithelial type II cells in primary culture

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human alveolar epithelial type II cells in primary culture
Creator	Fu, Wei He, Weiqun Li, Jianchun Li, Yimin Liu, Xiaoqing Mao, Pu Wu, Songling Zhang, Haibo Slutsky, Arthur
Source	PMC
abstract	Alveolar epithelial type II (AEII) cells are a key structure and defender in the lung but also are the targets in many lung diseases, including acute respiratory distress syndrome, ventilator-induced lung injury, and pulmonary fibrosis. We sought to establish an optimized method for high yielding and long maintenance of characteristics of primary human AEII cells to facilitate the investigation of the mechanisms of lung diseases at the cellular and molecular levels. Adult human peripheral normal lung tissues of oncologic patients undergoing lung resection were collected. The AEII cells were isolated and identified by the expression of pro-surfactant protein (SP)C, epithelial sodium channel (αENaC) and cytokeratin (CK)-8, the lamellar bodies specific for AEII cells, and confirmed by the histology using electron microscopy. The phenotype of AEII cells was characterized by the expression of surfactant proteins (SP-A, SP-B, SP-C, SP-D), CK-8, KL-6, αENaC, and aquaporin (AQP)-3, which was maintained over 20 days. The biological activity of the primary human AEII cells producing SP-C, cytokines, and intercellular adhesion molecule-1 was vigorous in response to stimulation with tumor necrosis factor-α. We have modified previous methods and optimized a method for isolation of high purity and long maintenance of the human AEII cell phenotype in primary culture. This method provides an important tool for studies aiming at elucidating the molecular mechanisms of lung diseases exclusively in AEII cells.
has issue date	2015-02-13(xsd:dateTime)
bibo:doi	10.14814/phy2.12288
bibo:pmid	25677546
has license	cc-by
sha1sum (hex)	e854283ba0d428f16fb5eec6a1ba210ff7925125
schema:url	https://doi.org/10.14814/phy2.12288
resource representing a document's title	Human alveolar epithelial type II cells in primary culture
has PubMed Central identifier	PMC4393197
has PubMed identifier	25677546
schema:publication	Physiol Rep
resource representing a document's body	covid:e854283ba0d428f16fb5eec6a1ba210ff7925125#body_text
is schema:about of	named entity 'normal' named entity 'collected' named entity 'This' named entity 'stimulation' named entity 'investigation' named entity 'defender' named entity 'primary culture' named entity 'alveolar' named entity 'SP-D' named entity 'DAYS' named entity 'VENTILATOR-INDUCED LUNG INJURY' named entity 'RESPONSE TO' named entity 'STIMULATION' named entity 'IMPORTANT' named entity 'MAINTENANCE' named entity 'PREVIOUS' named entity 'IDENTIFIED BY' named entity 'INVESTIGATION' named entity 'MECHANISMS' named entity 'LUNG' named entity 'HAVE' named entity 'AQUAPORIN' named entity 'BIOLOGICAL ACTIVITY' named entity 'CELLS' named entity 'ADULT' named entity 'ONCOLOGIC' named entity 'PATIENTS' named entity 'PERIPHERAL' named entity 'INCLUDING' named entity 'ELECTRON MICROSCOPY' named entity 'PROVIDES' named entity 'KEY' named entity 'PRODUCING' named entity 'EPITHELIAL SODIUM CHANNEL' named entity 'ACUTE RESPIRATORY DISTRESS SYNDROME' named entity 'HISTOLOGY' named entity 'LAMELLAR BODIES' named entity '29C' named entity 'LUNG RESECTION' named entity 'INTERCELLULAR ADHESION MOLECULE-1' named entity 'KL-6' named entity 'CYTOKERATIN' named entity 'TARGETS' named entity 'CK-8' named entity 'PRO-' named entity 'ESTABLISH' named entity 'ALVEOLAR' named entity 'TYPE II' named entity 'SURFACTANT' named entity 'TYPE II' named entity 'BUT' named entity 'EXPRESSION' named entity 'PRIMARY' named entity 'CELLULAR' named entity 'SPECIFIC' named entity 'SP-C' named entity 'TUMOR NECROSIS FACTOR' named entity 'HUMAN' named entity 'LONG' named entity 'EPITHELIAL' named entity 'PRIMARY CULTURE' named entity 'PULMONARY FIBROSIS' named entity 'PHENOTYPE' named entity 'LUNG DISEASES' named entity 'EPITHELIAL' named entity 'CELL PHENOTYPE' named entity 'ISOLATED' named entity 'SP-B' named entity 'LEVELS' named entity 'MODIFIED' named entity 'MOLECULAR' named entity 'METHODS' named entity 'STUDIES'

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