About: Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)

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About: Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)
Creator	Mizutani, Tetsuya Akashi, Hiroomi Fukushi, Shuetsu Morikawa, Shigeru Saijo, Masayuki Watanabe, Shumpei Yoshikawa, Yasuhiro Kurane, Ichiro Sakai, Kouji Kato, Kentaro Tohya, Yukinobu
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Emerging infectious diseases pose a significant risk to public health. Methods for rapid detection of pathogens are needed to effectively treat these diseases. Recently, we developed new methods for the rapid determination of viral RNA sequences, RDV ver1.0 and ver2.0. We demonstrated that these methods were able to simultaneously detect cDNA fragments of many different viruses without using sequence specific primers. However, some species of viruses, including the Yokose virus (YOKV), a flavivirus, could not be detected using the conventional procedures. OBJECTIVE: The RDV method was further modified to reduce the candidate PCR primer sets. STUDY DESIGN: Primer sets were reduced to 256 sets in the improved RDV ver3.0, and theoretically, all viral cDNA fragments ligated by two kinds of adaptors after digestion by two restriction enzymes could be amplified in the PCR step for direct sequencing. RESULTS: We succeeded in obtaining 118 YOKV cDNA fragments of the 141 sequence fragments. The cDNA fragments covered diverse range of viral genome. CONCLUSION: We were able to reduce the combinations of PCR primer sets used in the RDV method. This RDV method ver3.0 has a potential to detect viral cDNA fragments of both known and unknown RNA viruses rapidly and conveniently.
has issue date	2008-07-01(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2008.05.004
bibo:pmid	18595773
has license	no-cc
sha1sum (hex)	e9a86808a124d6f066162dda4c8b513e417bad2e
schema:url	https://doi.org/10.1016/j.jcv.2008.05.004
resource representing a document's title	Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)
has PubMed Central identifier	PMC7108420
has PubMed identifier	18595773
schema:publication	J Clin Virol
resource representing a document's body	covid:e9a86808a124d6f066162dda4c8b513e417bad2e#body_text
is schema:about of	named entity 'direct' named entity 'sets' named entity 'Study design' named entity 'determination' named entity 'effectively' named entity 'amplification' named entity 'effective' named entity 'VIRAL' named entity 'VIRUSES' named entity 'KNOWN' named entity 'SIGNIFICANT' named entity 'THESE' named entity 'DIGESTION' named entity 'POTENTIAL' named entity 'PROCEDURES' named entity 'CONVENTIONAL' named entity 'POSE' named entity 'RESTRICTION' named entity 'DETECTED' named entity 'MODIFIED' named entity 'RECENTLY' named entity 'STUDY DESIGN' covid:arg/e9a86808a124d6f066162dda4c8b513e417bad2e named entity 'ENZYMES' named entity 'CANDIDATE' named entity 'METHODS' named entity 'RISK' named entity 'AMPLIFICATION' named entity 'RAPID DETERMINATION' named entity 'RNA SEQUENCES' named entity 'EFFECTIVE' named entity 'LIGATION' named entity 'DIVERSE' named entity 'PCR' named entity 'NEEDED' named entity 'AMPLIFIED' named entity 'PRIMERS' named entity 'DETECTION' named entity 'COULD BE' named entity 'FRAGMENTS' named entity 'NEW' named entity 'CONCLUSION' named entity 'RAPID DETERMINATION' named entity 'FLAVIVIRUS' named entity 'PRIMER' named entity 'SPECIFIC' named entity 'TO DETECT' named entity 'SIMULTANEOUSLY' named entity 'RDV' named entity 'REDUCED' named entity 'TO REDUCE' named entity 'SUCCEEDED' named entity '118' named entity 'SEQUENCE' named entity 'VIRAL RNA' named entity 'MEDIATED' named entity 'COVERED' named entity 'UNKNOWN' named entity 'CDNA' named entity 'RAPID' named entity 'VIRAL GENOME' named entity 'OBJECTIVE' named entity 'KINDS' named entity 'RNA SEQUENCES' named entity 'USED' named entity 'DEMONSTRATED' named entity 'USING' named entity 'INCLUDING' named entity 'DIFFERENT' named entity 'EMERGING INFECTIOUS DISEASES' named entity 'VIRAL RNA'

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