About: Airway gene transfer using cationic emulsion as a mucosal gene carrier

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Airway gene transfer using cationic emulsion as a mucosal gene carrier Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Airway gene transfer using cationic emulsion as a mucosal gene carrier
Creator	Woo, Tae Chin, Ha Chung, Hesson Jeong, Seo Kwon, Ick Shin, Byung
Source	Medline; PMC
abstract	BACKGROUND: Delivery of genes to airway mucosa would be a very valuable method for gene therapy and vaccination. However, there have been few reports on suitable gene delivery systems for administration. In this study, we use a cationic emulsion system, which is physically stable and facilitates the transfer of genes in the presence of up to 90% serum, as a mucosal gene carrier. METHODS AND RESULTS: Cationic lipid emulsion was formulated with squalene and 1,2‐dioleoyl‐sn‐glycero‐3‐trimethylammoniumpropane (DOTAP) as major components. Emulsions formed stable complexes with DNA and protected and transferred DNA to target cells against DNase I digestion in the presence of mucosal destabilizers such as heparin sulfate (a polysaccharide of the glycosaminoglycan family in mucosa) and Newfectan (a natural lung extract of bovine) in an in vitro system. In contrast, commercial liposomes and counter liposomes, made with an identical lipid composition of emulsions, failed. After in vivo intranasal instillation, the cationic emulsion showed at least 200 times better transfection activity than the liposomal carriers in both nasal tissue and lung. CONCLUSIONS: These findings show that cationic emulsions can mediate gene transfection into airway epithelium, making it a good choice for transferring therapeutic genes and for genetic vaccination against an pathogenic infection via an airway route. Copyright © 2005 John Wiley & Sons, Ltd.
has issue date	2005-05-31(xsd:dateTime)
bibo:doi	10.1002/jgm.711
bibo:pmid	15929068
has license	no-cc
sha1sum (hex)	e9aae8ed1bd858d61f75e26dc64dcc47dc83be46
schema:url	https://doi.org/10.1002/jgm.711
resource representing a document's title	Airway gene transfer using cationic emulsion as a mucosal gene carrier
has PubMed Central identifier	PMC7167195
has PubMed identifier	15929068
schema:publication	J Gene Med
resource representing a document's body	covid:e9aae8ed1bd858d61f75e26dc64dcc47dc83be46#body_text
is schema:about of	named entity 'However' covid:arg/e9aae8ed1bd858d61f75e26dc64dcc47dc83be46 named entity 'systems' named entity 'gene therapy' named entity 'gene delivery' named entity 'mucosal' named entity 'gene transfer' named entity 'anesthetized' named entity 'transfection' named entity 'liposome' named entity 'intranasal' named entity 'emulsion' named entity 'enzymatic digestion' named entity 'lipid emulsion' named entity 'mucosal' named entity 'Mucosal' named entity 'L-aspartic acid' named entity 'dose-response' named entity 'mucosal surfaces' named entity 'Tween 80' named entity 'polydispersity' named entity 'homogenized' named entity 'negative charge' named entity 'proteoglycan' named entity 'photon' named entity 'DNase' named entity 'polysaccharide' named entity 'C/D' named entity 'glycosaminoglycan' named entity 'Lipofectamine' named entity 'gene therapy' named entity 'liposome' named entity 'lipid emulsion' named entity 'protein' named entity 'emulsion' named entity 'phosphoethanolamine' named entity 'lymphoid tissue' named entity 'phenol' named entity 'emulsion' named entity 'gene carrier' named entity '1, 2' named entity 'heparin sulfate' named entity 'emulsion' named entity 'nasal cavity' named entity 'transfected' named entity 'fetal bovine serum' named entity 'Tween 80' named entity 'serum' named entity 'squalene' named entity 'Naked DNA' named entity 'emulsion' named entity 'mucosal' named entity 'transgene' named entity 'Luciferase' named entity 'liposomes' named entity 'mucosal' named entity 'immune response' named entity 'sonicator' named entity 'severe acute respiratory syndrome coronavirus' named entity 'transfection' named entity 'agarose gel' named entity 'emulsion' named entity 'luciferase' named entity 'steric' named entity 'staining' named entity 'emulsion' named entity 'morphology' named entity 'administrated' named entity 'intranasal' named entity 'transfected' named entity 'mucosal' named entity 'Tween 80' named entity 'lipid' named entity 'liposome' named entity 'heparin' named entity 'intravenous administration'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software