About: Development of an indirect ELISA for detecting porcine deltacoronavirus IgA antibodies

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About: Development of an indirect ELISA for detecting porcine deltacoronavirus IgA antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of an indirect ELISA for detecting porcine deltacoronavirus IgA antibodies
Creator	Shi, Hongyan Liu, Jianbo Wang, Xiaobo Zhang, Jialin Liu, Qiuge Lu, Manman Chen, · Zhang, · Feng, · Da Shi, ·
Source	Medline; PMC
abstract	Porcine deltacoronavirus (PDCoV) is a novel coronavirus that can cause vomiting and watery diarrhea in pigs and death in piglets. Since PDCoV was first detected in 2009 in Hong Kong, the prevalence of PDCoV has increased in recent years, resulting in serious economic losses to the swine industry. The coronavirus spike (S) protein is an antigen that has been demonstrated to contain epitopes that induce neutralizing antibodies. The presence of serum and milk IgA antibodies against pathogens that replicate primarily on mucosal surfaces is important for mucosal immunity. Here, an indirect anti-PDCoV IgA antibody enzyme-linked immunosorbent assay (PDCoV S1 IgA ELISA) using the purified S1 portion of S protein as the coating antigen was developed to detect PDCoV IgA antibodies in serum and sow’s milk. A receiver operating characteristic (ROC) curve analysis showed high specificity and sensitivity of the PDCoV-S1-IgA-ELISA based on samples confirmed by IFA. Anti-PDCoV IgA antibodies in 152 serum samples and 65 milk samples collected from six farms that had experienced diarrhea outbreaks within previous last two years were detected by this assay, and 62.5% of the serum samples and 100% of the milk samples were positive for PDCoV. The indirect ELISA method established in this study will provide a convenient tool for measurement of serum and milk IgA levels against PDCoV in pig herds, rapid detection of PDCoV infection in pigs, and evaluation of the immunogenicity of vaccines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04541-6) contains supplementary material, which is available to authorized users.
has issue date	2020-02-12(xsd:dateTime)
bibo:doi	10.1007/s00705-020-04541-6
bibo:pmid	32052195
has license	no-cc
sha1sum (hex)	e9c85c5f11af75c0dfe0e4d5a7d471568fd90236
schema:url	https://doi.org/10.1007/s00705-020-04541-6
resource representing a document's title	Development of an indirect ELISA for detecting porcine deltacoronavirus IgA antibodies
has PubMed Central identifier	PMC7087096
has PubMed identifier	32052195
schema:publication	Arch Virol
resource representing a document's body	covid:e9c85c5f11af75c0dfe0e4d5a7d471568fd90236#body_text
is schema:about of	named entity 'receiver operating characteristic' named entity 'vomiting' named entity 'antibodies' named entity 'milk' named entity 'enzyme-linked immunosorbent assay' named entity 'provide' named entity 'vaccines' named entity 'pigs' named entity 'diarrhea' named entity 'deltacoronavirus' named entity 'Here' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'MEASUREMENT' named entity 'WATERY DIARRHEA' named entity 'PORCINE DELTACORONAVIRUS' named entity 'ANTIBODIES' named entity 'PROTEIN ' named entity 'SAMPLES' named entity 'CONFIRMED BY' named entity 'IMMUNOGENICITY' named entity 'RECEIVER' named entity '28S' named entity 'CURVE' named entity 'CHARACTERISTIC' named entity 'MILK' named entity 'EXPERIENCED' named entity 'ECONOMIC' named entity 'INDUCE' named entity 'CAUSE' named entity 'IS A' named entity 'MUCOSAL' named entity 'MUCOSAL IMMUNITY' named entity 'serum' named entity 'herds' named entity 'samples' named entity 'sensitivity' named entity 'IgA' named entity 'indirect' named entity 'demonstrated' named entity 'industry' named entity 'detect' named entity 'protein' named entity 'rapid' named entity 'evaluation' named entity 'samples' named entity 'antibody' named entity 'epitopes' named entity 'spike (S) protein' named entity 'watery diarrhea' named entity 'antigen' named entity 'immunogenicity' named entity 'milk' named entity 'IgA antibodies' named entity 'IgA antibodies' named entity 'IgA' named entity 'Laos' named entity 'PBS' named entity 'PBS' named entity 'infection' named entity 'IgA' named entity 'substrate' named entity 'coronaviruses' named entity 'GenBank' named entity 'protease' named entity '1:500' named entity 'DAPI' named entity 'tissue culture' named entity 'Deltacoronavirus' named entity 'PEDV' named entity 'pathogen' named entity 'protein' named entity 'Microplate Reader'

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