About: Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium

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About: Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium
Creator	Kota, Krishna Batra, Harshvardhan Makam, Sathyanarayan Prakash, Narayana Reddy, Rohini, & Shivakiran, & Tuteja, Urmil Urs, Radhika Yatung, Gyati
Source	Medline; PMC
abstract	In the present study, we have generated a murine monoclonal antibody (mAb) named Sal-06 by using the crude outer membrane protein preparation of Salmonella enteric subsp. enterica serovar Typhimurium ATCC 14028 strain as antigen. Sal-06mAb belonging to IgG1 isotype demonstrated broad cross-reactivity to standard and isolated strains of genus Salmonella and others such as Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis. Cross-reactivity across several bacterial genera indicated that the epitopes reactive to Sal-06mAb are conserved among these members. Neutralizing effects of Sal-06mAb on Salmonella growth and survival was evaluated in vitro using bacteriostatic and bactericidal activity with and without complement and bacterial invasion inhibition assay. Sal-06mAb demonstrated a bacteriostatic effect on the growth of S. typhimurium ATCC 14028 strain which is both time and concentration (of mAb) dependent. It was also found that the bacterial growth inhibition was complement independent. When the bacterial cells were preincubated with Sal-06mAb, it reduced the adherence and invasion of bacterial cells into A549 epithelial cell line. This was confirmed by CFU count analysis, phase contrast, and fluorescence microscopy. Scanning electron microscope (SEM) imaging confirmed the antimicrobial effects of Sal-06mAb on S. typhimurium ATCC 14028. The development of broadly reactive and cross protective Sal-06mAb opens new possibilities for immunotherapy of sepsis caused by Gram-negative Enterobacteriaceae members.
has issue date	2020-01-29(xsd:dateTime)
bibo:doi	10.1007/s00253-020-10394-5
bibo:pmid	31997109
has license	no-cc
sha1sum (hex)	eaad43de1538db306ca619b37bab8cd61964423d
schema:url	https://doi.org/10.1007/s00253-020-10394-5
resource representing a document's title	Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium
has PubMed Central identifier	PMC7080182
has PubMed identifier	31997109
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:eaad43de1538db306ca619b37bab8cd61964423d#body_text
is schema:about of	named entity 'DIRECTED' named entity 'OUTER MEMBRANE PROTEIN' named entity 'demonstrated' named entity 'ATCC' named entity 'Klebsiella' named entity 'reactive' named entity 'Sal' named entity 'bacterial' named entity 'complement' named entity 'MONOCLONAL ANTIBODY' named entity 'EFFECTS' named entity 'INHIBITION' named entity 'IGG1' named entity 'SEPSIS' named entity 'COMPLEMENT' named entity 'S. TYPHIMURIUM' named entity 'REACTIVE' named entity 'DEPENDENT' named entity 'BACTERIAL GROWTH' named entity 'ENTERIC' named entity 'DEMONSTRATED' named entity 'CFU' named entity 'PHASE CONTRAST' named entity 'SALMONELLA' named entity 'ESCHERICHIA COLI' named entity 'INDEPENDENT' named entity 'CONSERVED' named entity 'A549' named entity 'CELLS' named entity 'CROSS' covid:arg/eaad43de1538db306ca619b37bab8cd61964423d named entity 'SALMONELLA TYPHIMURIUM' named entity 'PROTEIN ' named entity 'FUNCTIONAL' named entity 'CHARACTERIZATION' named entity 'ATCC' named entity 'REDUCED' named entity 'IN VITRO' named entity 'STUDY' named entity 'BELONGING' named entity 'ISOTYPE' named entity 'NEGATIVE' named entity 'INDICATED' named entity 'CAUSED BY' named entity 'EVALUATED' named entity 'OPENS' named entity 'SEROVAR' named entity 'ISOLATED' named entity 'CONFIRMED BY' named entity 'PROTEIN PREPARATION' named entity 'DEVELOPMENT' named entity 'HAVE' named entity 'ADHERENCE' named entity 'ANALYSIS' named entity 'SCANNING ELECTRON MICROSCOPE' named entity 'STANDARD' named entity 'CONFIRMED' named entity 'ANTIGEN' named entity 'IMAGING' named entity 'GENUS' named entity 'BROAD' named entity 'REACTIVITY' named entity 'EPITHELIAL CELL LINE' named entity 'COUNT' named entity 'BACTERICIDAL ACTIVITY' named entity 'FLUORESCENCE MICROSCOPY' named entity 'CONCENTRATION' named entity 'SAL' named entity 'MAB' named entity 'MEMBERS' named entity 'OUTER MEMBRANE PROTEIN' named entity 'BACTERIAL'

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