About: MicroRNA expression profiling in PBMCs of Indian water Buffalo (Bubalus bubalis) infected with Brucella and Johne’s disease

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About: MicroRNA expression profiling in PBMCs of Indian water Buffalo (Bubalus bubalis) infected with Brucella and Johne’s disease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MicroRNA expression profiling in PBMCs of Indian water Buffalo (Bubalus bubalis) infected with Brucella and Johne’s disease
Creator	Choudhary, Ratan Dhanoa, Jasdeep Kaur, Simarjeet Mukhopadhyay, Chandra Sethi, Ram Singh, Amarjit Singh, Jasdeep
Source	PMC
abstract	BACKGROUND: MicroRNAs play key roles in host-pathogen-interactions and disease pathogenesis. Our aim was to characterize the differentially expressed miRNAs in the blood cells of diseased (Brucellosis-positive, Johne’s disease-positive) and healthy- water buffaloes. The pooled small-RNA samples of each group were sequenced on Ion Torrent Personal Genome Machine (PGM) sequencer and the data were analyzed for differential expression. RESULTS: Here we identified 274 known miRNAs with bovine homologs and 36 novel mature-star miRNAs from the sequnces of small RNA libraries. Overall 195 miRNAs were common to all the three groups. Certain miRNAs such as bta-miR-21-5p, −26a, −29a/b, −30d − 103, − 140, − 150, − 191, − 374, − 1434-5p,-1260b, − 2484 and let-7 members were abundantly expressed in diseased groups. Bta-miR-1434-5p, − 188, −200c were up-regulated (> 1.5 folds) while bta-miR-27a-5p, −34b and -2285x were down-regulated (> 100 folds) in Brucellosis group. In Johne’s Disease group, only 3 miRNAs (bta-miR-1434-5p, − 2340 and − 2484) were up-regulated (> 1.5 folds). The functional classification of miRNA target genes into gene ontology (GO) terms indicated their involvement in innate immunity and cellular process of disease pathogenesis. Expression profile of four differentially expressed miRNAs (bta-miR-9-5p, − 677, − 331-3p and − 2440) and eight predicted target-genes were validated through reverse transcriptase qPCR. CONCLUSION: This study provides a valuable frame of reference for elucidation of regulatory roles of miRNAs associated with disease pathogenesis in water buffaloes as well as identification of miRNA biomarkers for disease diagnosis and treatment.
has issue date	2020-05-22(xsd:dateTime)
bibo:doi	10.1186/s41544-020-00049-y
has license	cc-by
sha1sum (hex)	eaaec98aade29d0f1f92449fb55e68301601a8da
schema:url	https://doi.org/10.1186/s41544-020-00049-y
resource representing a document's title	MicroRNA expression profiling in PBMCs of Indian water Buffalo (Bubalus bubalis) infected with Brucella and Johne’s disease
has PubMed Central identifier	PMC7242893
schema:publication	ExRNA
resource representing a document's body	covid:eaaec98aade29d0f1f92449fb55e68301601a8da#body_text
is schema:about of	named entity 'samples' named entity 'PBMCs' named entity 'disease' covid:arg/eaaec98aade29d0f1f92449fb55e68301601a8da named entity 'Our' named entity 'pathogenesis' named entity 'host-pathogen' named entity 'Indian water Buffalo' named entity 'Johne's disease' named entity 'down-regulated' named entity 'immune responses' named entity 'bovine mastitis' named entity 'bta' named entity 'miRNAs' named entity 'aseptically' named entity 'cell proliferation' named entity 'miRNA' named entity 'beta-actin' named entity 'Brucella' named entity 'miR' named entity 'gene ontology' named entity 'spleen' named entity 'primers' named entity 'infection' named entity 'miRNAs' named entity 'miRNAs' named entity 'miRNAs' named entity 'gene ontology' named entity 'Brucellosis' named entity 'E. coli infections' named entity 'bacteria' named entity 'sRNA' named entity 'control group' named entity 'receptor' named entity 'bta' named entity 'fecal matter' named entity 'Brucella melitensis' named entity 'down-regulated' named entity 'M. paratuberculosis' named entity 'water buffaloes' named entity 'M. paratuberculosis' named entity 'gene expression' named entity 'reproductive organs' named entity 'EDTA' named entity 'bta' named entity 'immune functions' named entity 'microRNAs' named entity 'Bos taurus' named entity 'bta' named entity 'infection' named entity 'livestock' named entity 'PCR' named entity 'mycobacterial' named entity 'bta' named entity 'one-way ANOVA' named entity 'miRNAs' named entity 'gene' named entity 'pre-miRNA' named entity 'CPSF2' named entity 'experimental groups' named entity 'miRNA' named entity 'beta-actin' named entity 'endogenous control' named entity 'RNA' named entity 'sRNA' named entity 'differentially expressed' named entity 'infection' named entity 'lymph nodes' named entity 'miRNAs' named entity 'miRNA' named entity 'cDNA libraries' named entity 'miRNA' named entity 'buffalo' named entity 'microarrays' named entity 'qPCR' named entity 'India' named entity 'miRNA'

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