About: Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS

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About: Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS
Creator	Gong, Zuojiong Wu, Zhenghui Ye, Li Ye, Linbai Zhu, Ying Timani, Khalid
Source	Elsevier; Medline; PMC
abstract	Abstract A novel coronavirus has been associated with a worldwide outbreak of atypical pneumonia referred to as Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV nucleocapsid (N) protein has been cloned sequenced and expressed in Escherichia coli strain. Purified N protein was used to measure the SARS-CoV specific IgG antibodies from 16 SARS-CoV infected patients’ sera and from 131 control subjects using ELISA assay. Specific antibody responses to the purified recombinant N protein after 10, 20, and 30 days of disease onset were observed in 13 of 16 (81.3%), 16 of 16 (100%) and 16 of 16 (100%) SARS patients sera, respectively. Comparison of detection results with a commercially available diagnostic kit coated with a mixture of SARS-CoV viral proteins showed 9 of 16 (56.3%), 13 of 16 (81.3%), and 15 of 16 (93.7%) positive responses, respectively. None of 131 control sera gave positive reaction in either assay. This data suggests that the N protein of SARS-CoV is immunodominant and this ELISA based test assay for detecting the SARS-CoV N antigen may hold a significant value for SARS diagnosis.
has issue date	2004-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2004.01.001
bibo:pmid	15163419
has license	els-covid
sha1sum (hex)	eb1f3f98b2e81a0248b37c0123d4cca0b4913d9c
schema:url	https://doi.org/10.1016/j.jcv.2004.01.001
resource representing a document's title	Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS
has PubMed Central identifier	PMC7130062
has PubMed identifier	15163419
schema:publication	Journal of Clinical Virology
resource representing a document's body	covid:eb1f3f98b2e81a0248b37c0123d4cca0b4913d9c#body_text
is schema:about of	named entity 'CLONING' named entity 'responses' named entity 'purified' named entity 'control' named entity 'diagnosis' named entity 'disease' named entity 'IgG' named entity 'PURIFICATION' named entity 'DETECTION' named entity 'SUBJECTS' named entity 'TEST' named entity 'VALUE' named entity '10%' named entity 'ELISA' named entity 'DAYS' named entity 'SIGNIFICANT' named entity 'N ANTIGEN' named entity 'PATIENTS' covid:arg/eb1f3f98b2e81a0248b37c0123d4cca0b4913d9c named entity 'USED' named entity 'VIRAL PROTEINS' named entity 'PROTEIN ' named entity 'NOVEL CORONAVIRUS' named entity '28100' named entity 'IGG' named entity 'OBSERVED' named entity 'DETECTING' named entity 'POSITIVE' named entity 'BASED' named entity 'HOLD' named entity 'INFECTED' named entity 'CONTROL' named entity 'DISEASE ONSET' named entity 'GAVE' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'SERODIAGNOSIS' named entity 'EXPRESSION' named entity 'ANTIBODIES' named entity 'WORLDWIDE' named entity 'OUTBREAK' named entity 'ELISA ASSAY' named entity 'EXPRESSED' named entity 'ASSOCIATED WITH' named entity 'RECOMBINANT' named entity 'REACTION' named entity 'NUCLEOCAPSID PROTEIN' named entity 'MIXTURE' named entity 'REFERRED TO' named entity 'ATYPICAL PNEUMONIA' named entity 'USING' named entity 'SARS' named entity 'SEQUENCING' named entity 'COMPARISON' named entity 'SARS' named entity 'DIAGNOSTIC' named entity 'N PROTEIN' named entity 'SARS-COV' named entity 'NUCLEOCAPSID' named entity 'EITHER' named entity 'RESULTS' named entity 'PURIFIED' named entity 'KIT' named entity 'RESPONSES' named entity '2856' named entity '20%' named entity 'CLONED' named entity 'SPECIFIC' named entity 'SERA' named entity 'DATA' named entity 'ASSAY' named entity 'ESCHERICHIA COLI' named entity 'TO MEASURE' named entity 'COATED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'PROTEIN ' named entity 'AVAILABLE'

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