About: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

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About: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing
Creator	Wang, Li Li, Kai Jiang, Jing Hu, Zheng Chen, Yonglong Li, Xiangning Luo, Dixian Shi, Zhaoying
source	PMC
abstract	Overlap extension polymerase chain reaction (PCR) is a powerful technology for DNA assembly. Based on this technology, we synthesized DNA templates, which were transcribed into sgRNA in vitro, and further detected their efficiency of purified sgRNAs with Cas9 nuclease. The sgRNAs synthesized by this approach can effectively cleave the DNA fragments of interest in vitro and in vivo. Compared with the conventional method for generating sgRNA, it does not require construction of recombinant plasmids and design of primers to amplify sgRNA core fragment. Only several short primers with overlapped sequences are needed to assemble a DNA fragment as the template of sgRNA. This modified and simplified method is highly applicable and less time-consuming.
has issue date	2019-10-24(xsd:dateTime)
bibo:doi	10.1186/s13578-019-0350-7
bibo:pmid	31673328
has license	cc-by
sha1sum (hex)	eb9a77c49a82ea14701be517ad9792664b82660c
schema:url	https://doi.org/10.1186/s13578-019-0350-7
resource representing a document's title	Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing
has PubMed Central identifier	PMC6814055
has PubMed identifier	31673328
schema:publication	Cell Biosci
resource representing a document's body	covid:eb9a77c49a82ea14701be517ad9792664b82660c#body_text
is schema:about of	named entity 'plasmids' named entity 'sgRNA' named entity 'modified' named entity 'primers' named entity 'sgRNA' named entity 'primers' named entity 'application' named entity 'HIGHLY' named entity 'PRIMERS' named entity 'SGRNA' named entity 'CUSTOMIZED' named entity 'TEMPLATES' named entity 'OVERLAPPING' named entity 'USING' named entity 'fragment' named entity 'generating' named entity 'PCR' named entity 'recombinant' named entity 'Cas9' named entity 'This' named entity 'gene' named entity 'Cas9' named entity 'sgRNA' named entity 'sgRNA' named entity 'Xenopus tropicalis' named entity 'ZFN' named entity 'sgRNA' named entity 'repair mechanisms' named entity 'Clustered Regularly Interspaced Short Palindromic Repeats' named entity 'DNA double strand breaks' named entity 'targeting sequences' named entity 'sgRNA' named entity 'transcription' named entity 'X. tropicalis' named entity 'transcription' named entity 'loci' named entity 'CRISPR' named entity 'indels' named entity 'transcription' named entity 'primers' named entity 'plasmid' named entity 'primers' named entity 'gene' named entity 'digestion' named entity 'genomic DNA' named entity 'transcription' named entity 'long primer' named entity 'assay' named entity 'target site' named entity 'transcription' named entity 'nuclease' named entity 'primers' named entity 'Cas9' named entity 'sgRNA' named entity 'Znf238' named entity 'gene' named entity 'digestion' named entity 'primer extension' named entity 'transcription' named entity 'sgRNA' named entity 'sgRNA' named entity 'PCR amplification' named entity 'synthesize DNA' named entity 'sgRNA' named entity 'EGFR' named entity 'mutation analysis' named entity 'targeting sequences' named entity 'digestion' named entity 'DNA sequencing' named entity 'Cas9' named entity 'DNA sequencing' named entity 'primers' named entity 'sgRNA' named entity 'gRNAs'

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