About: A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus

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About: A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus
Creator	Bergeron, Éric Flint, Mike Shrivastava-Ranjan, Punya Chatterjee, Payel Lo, Michael Nichol, Stuart Spiropoulou, Christina Lin, David Mcmullan, Laura Tai, Andrew Welch, Stephen
source	Medline; PMC
abstract	There are no approved therapies for Ebola virus infection. Here, to find potential therapeutic targets, we perform a screen for genes essential for Ebola virus (EBOV) infection. We identify GNPTAB, which encodes the α and β subunits of N-acetylglucosamine-1-phosphate transferase. We show that EBOV infection of a GNPTAB knockout cell line is impaired, and that this is reversed by reconstituting GNPTAB expression. Fibroblasts from patients with mucolipidosis II, a disorder associated with mutations in GNPTAB, are refractory to EBOV, whereas cells from their healthy parents support infection. Impaired infection correlates with loss of the expression of cathepsin B, known to be essential for EBOV entry. GNPTAB activity is dependent upon proteolytic cleavage by the SKI-1/S1P protease. Inhibiting this protease with the small-molecule PF-429242 blocks EBOV entry and infection. Disruption of GNPTAB function may represent a strategy for a host-targeted therapy for EBOV.
has issue date	2019-01-17(xsd:dateTime)
bibo:doi	10.1038/s41467-018-08135-4
bibo:pmid	30655525
has license	cc-by
sha1sum (hex)	ec432ab2fea7d620db7138b7c72ceecf03f09785
schema:url	https://doi.org/10.1038/s41467-018-08135-4
resource representing a document's title	A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus
has PubMed Central identifier	PMC6336797
has PubMed identifier	30655525
schema:publication	Nat Commun
resource representing a document's body	covid:ec432ab2fea7d620db7138b7c72ceecf03f09785#body_text
is schema:about of	named entity 'infection' named entity 'disorder' named entity 'CRISPR' named entity 'ACTIVITY' named entity 'ESSENTIAL' named entity 'PROTEOLYTIC' named entity 'ASSOCIATED WITH' named entity 'CATHEPSIN B' named entity 'PF-429242' named entity 'STRATEGY' named entity 'IMPAIRED' named entity 'SCREEN' named entity 'KNOCKOUT CELL' named entity 'LOSS OF' named entity 'CELL LINE' named entity 'ENTRY' named entity 'INHIBITING' named entity 'DISRUPTION' named entity 'MOLECULE' named entity 'DEPENDENT' named entity 'PARENTS' named entity 'MUTATIONS' named entity 'MUCOLIPIDOSIS II' named entity 'GNPTAB' named entity 'FUNCTION' named entity 'PATIENTS' named entity 'INFECTION' named entity 'RECONSTITUTING' named entity 'SUPPORT' named entity 'HEALTHY' named entity 'TARGETS' named entity 'IDENTIFY' named entity 'THEIR' named entity 'EBOLA VIRUS' named entity 'SCREEN' named entity 'CELLS' named entity 'N-ACETYLGLUCOSAMINE-1-PHOSPHATE TRANSFERASE' named entity 'ANTIVIRAL' named entity 'TARGET' named entity 'CRISPR' named entity 'APPROVED' named entity 'EBOLA VIRUS INFECTION' named entity 'THERAPIES' named entity 'EXPRESSION' named entity 'EBOLA VIRUS' named entity 'REFRACTORY' named entity 'FIBROBLASTS' named entity 'BLOCKS' named entity 'HOST' named entity 'PROTEASE' named entity 'DISORDER ASSOCIATED' named entity 'CLEAVAGE' named entity 'REVERSED' named entity 'N-ACETYLGLUCOSAMINE-1-PHOSPHATE TRANSFERASE' named entity 'SKI-1' named entity 'GENOME' named entity 'TARGETED THERAPY' named entity 'SMALL' named entity 'WIDE' named entity 'POTENTIAL' named entity 'HERE' named entity 'GENES' named entity 'THERAPEUTIC' named entity 'KNOWN' named entity 'PERFORM' named entity 'POTENTIAL' named entity 'REPRESENT' covid:arg/ec432ab2fea7d620db7138b7c72ceecf03f09785 named entity 'screen' named entity 'loss' named entity 'Mucolipidosis'

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