About: Open Reading Frame 8a of the Human Severe Acute Respiratory Syndrome Coronavirus Not Only Promotes Viral Replication but Also Induces Apoptosis

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About: Open Reading Frame 8a of the Human Severe Acute Respiratory Syndrome Coronavirus Not Only Promotes Viral Replication but Also Induces Apoptosis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Open Reading Frame 8a of the Human Severe Acute Respiratory Syndrome Coronavirus Not Only Promotes Viral Replication but Also Induces Apoptosis
Creator	Lien, Te-Cheng Chen, Chia-Yen Lee, Yuan-Ming Chen, Kuan-Hsuan Chen, Yi-Ming Jap, Tjin-Shing Chan, Yu-Juin Kao, Lung-Sen Lee, Hsin-Chen Lin, Chi-Hung Ping, Yueh-Hsin
Source	Medline; PMC
abstract	Background. A unique genomic difference between human and civet severe acute respiratory syndrome coronaviruses (SARS-CoVs) is that the former has a deletion of 29 nucleotides from open reading frame (orf) 8d that results in the generation of orf8a and orf8b. The objectives of the present study were to analyze antibody reactivity to ORF8a in patients with SARS and to elucidate the function of ORF8a. Methods. Western-blot and immunofluorescent antibody assays were used to detect anti-ORF8a antibody. SARS-CoV HKU39849 was used to infect stable clones expressing ORF8a and cells transfected with small interfering RNA (siRNA). The virus loads (VLs) and cytopathic effects (CPEs) were recorded. Confocal microscopy and several mitochondria-related tests were used to study the function of ORF8a. Results. Two (5.4%) of 37 patients with SARS had anti-ORF8a antibodies. The VLs in the stable clones expressing ORF8a were significantly higher than those in control subjects 5 days after infection. siRNA against orf8a significantly reduced VLs and interrupted the CPE. ORF8a was found to be localized in mitochondria, and overexpression resulted in increases in mitochondrial transmembrane potential, reactive oxygen species production, caspase 3 activity, and cellular apoptosis. Conclusions. ORF8a not only enhances viral replication but also induces apoptosis through a mitochondria-dependent pathway.
has issue date	2007-08-01(xsd:dateTime)
bibo:doi	10.1086/519166
bibo:pmid	17597455
has license	no-cc
sha1sum (hex)	ec94cfc15d3eb626d9ce7f9e8251e8aece944fb5
schema:url	https://doi.org/10.1086/519166
resource representing a document's title	Open Reading Frame 8a of the Human Severe Acute Respiratory Syndrome Coronavirus Not Only Promotes Viral Replication but Also Induces Apoptosis
has PubMed Central identifier	PMC7204190
has PubMed identifier	17597455
schema:publication	J Infect Dis
resource representing a document's body	covid:ec94cfc15d3eb626d9ce7f9e8251e8aece944fb5#body_text
is schema:about of	named entity 'antibody' named entity 'coronaviruses' named entity 'deletion' named entity 'Not' named entity 'Human' named entity 'severe acute respiratory syndrome' named entity 'plasmid DNA' named entity 'clone' named entity 'clone' named entity 'SARS-CoV' named entity 'Tat protein' named entity 'protein' named entity 'positively charged' named entity 'keyhole limpet hemocyanin' named entity 'Molecular Probes' named entity 'National Center for Biotechnology Information' named entity 'GST' named entity 'mRNA expression' named entity 'RT-PCR' named entity 'Applied Biosystems' named entity 'fluorochrome' named entity 'cultured cells' named entity 'mitochondria' named entity 'nucleocapsid' named entity 'protein' named entity 'lysates' named entity 'cDNA' named entity 'N-terminal region' named entity 'cell lines' named entity 'cell cultures' named entity 'SARS-CoV' named entity 'viral genomes' named entity 'Epstein-Barr virus' named entity 'N-terminal region' named entity 'enzyme' named entity 'pH 7' named entity '293T cells' named entity 'pathogenesis' named entity 'SARS' named entity 'GST' named entity 'oligonucleotides' named entity 'GFP' named entity 'trypsinized' named entity 'PBS' named entity 'chemically synthesized' named entity 'subcellular localization' named entity 'antiserum' named entity 'plasmid DNA' named entity 'viral replication' named entity 'apoptosis' named entity 'supernatants' named entity 'SARS-CoV' named entity 'glycerol' named entity 'clone' named entity 'Applied Biosystems' named entity 'serum' named entity 'FACS' named entity 'kDa' named entity 'transfected' named entity 'apoptosis' named entity 'Mitochondria' named entity 'nucleocapsid proteins' named entity 'motif' named entity 'GST' named entity 'DMEM' named entity 'gene' named entity 'RT-PCR' named entity 'mitochondria' named entity 'Taipei' named entity 'antibody' named entity 'Kelowna'

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