About: RNA recombination in the genome of Barley stripe mosaic virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA recombination in the genome of Barley stripe mosaic virus
Creator	Edwards, M Jackson+, And Perrv, I
Source	Elsevier; Medline; PMC
abstract	Abstract Barley stripe mosaic Hordeivirus (BSMV) is a positive-strand RNA virus requiring three single-stranded RNAs (α, β, and γ) for infectivity. A terminal-sequence-dependent cloning strategy was used to clone the entire genome of the CV17 strain. Full-length γ cDNA clones were obtained when oligonucleotides specific for the 5′-terminal sequence of RNA α were used in the cloning procedure, but not when RNA γ-specific oligonucleotides were used. Sequence analysis of six putative γ cDNA clones revealed that nucleotides 1–70 possess 89% homology with the first 70 nucleotides of RNA α. This leader region is separated from the γ-specific coding region by an eight-base intervening sequence common to both CV17 RNAs α and γ. Northern and Southern hybridization with oligonucleotide probes specific for either α or γ leader sequences indicated that CV17 γ cDNA clones are representative of native CV17 γ RNAs. Furthermore, bioassays indicated that in vitro transcripts derived from these γ cDNA clones were infectious when coinoculated with in vitro transcripts of full-length α and β cDNA clones. Thus, the evidence suggests that RNA γ of BSMV strain CV17 is a recombinant molecule which may have arisen as a result of natural recombination between RNAs α and γ.
has issue date	1992-07-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(92)90722-2
bibo:pmid	1604824
has license	els-covid
sha1sum (hex)	ec9a51accf3afe859de847c59a0e50bb0be2968e
schema:url	https://doi.org/10.1016/0042-6822%2892%2990722-2
resource representing a document's title	RNA recombination in the genome of Barley stripe mosaic virus
has PubMed Central identifier	PMC7173315
has PubMed identifier	1604824
schema:publication	Virology
resource representing a document's body	covid:ec9a51accf3afe859de847c59a0e50bb0be2968e#body_text
is schema:about of	named entity 'cDNA clones' named entity 'Thus' named entity 'strain' named entity 'Mosaic' named entity 'Barley' named entity 'recombination' named entity 'cDNA clones' named entity 'RNA' named entity 'oligonucleotides' named entity 'natural' named entity 'This' named entity 'homology' named entity 'revealed' named entity 'oligonucleotides' named entity 'clones' named entity 'oligonucleotides' named entity 'Sequence analysis' named entity 'cDNA clones' named entity 'nucleotides' named entity 'Hordeivirus' named entity 'entire genome' named entity 'BSMV' named entity 'gel electrophoresis' named entity '3.8' named entity 'RNA' named entity 'recombination' named entity 'oligonucleotide' named entity 'ssDNA' named entity 'cDNA' named entity 'dideoxynucleotide' named entity 'gene' named entity 'RNA' named entity 'RNA' named entity 'bacteriophage' named entity 'RNAs' named entity 'polypeptides' named entity 'infectivity' named entity 'RNA' named entity 'barley' named entity 'hybridization' named entity 'cDNA clones' named entity 'RNAs' named entity 'Southern blots' named entity 'ND1' named entity 'Virion' named entity 'cDNA' named entity 'genetic drift' named entity '32P' named entity 'cDNA clones' named entity 'primer' named entity 'protoplasts' named entity 'coding regions' named entity 'leader sequence' named entity 'leader sequence' named entity 'Northern blot' named entity 'recombination' named entity 'cDNA clones' named entity 'RNAs' named entity 'infection' named entity 'recombinant' named entity 'ORF' named entity 'sequence conservation' named entity 'primer' named entity 'recombinant' named entity 'cDNA' named entity 'cloning' named entity 'transcription' named entity 'sequence divergence' named entity 'divergence' named entity 'single-stranded' named entity 'RNA' named entity 'RNA' named entity 'BSMV' named entity 'RNA' named entity 'RNA viruses' named entity 'Hordeivirus' named entity 'cDNA clone' named entity 'primer' named entity 'Inoculated'

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