About: Mucosal Vaccination with Recombinant Lactobacillus casei-Displayed CTA1-Conjugated Consensus Matrix Protein-2 (sM2) Induces Broad Protection against Divergent Influenza Subtypes in BALB/c Mice

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Mucosal Vaccination with Recombinant Lactobacillus casei-Displayed CTA1-Conjugated Consensus Matrix Protein-2 (sM2) Induces Broad Protection against Divergent Influenza Subtypes in BALB/c Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mucosal Vaccination with Recombinant Lactobacillus casei-Displayed CTA1-Conjugated Consensus Matrix Protein-2 (sM2) Induces Broad Protection against Divergent Influenza Subtypes in BALB/c Mice
Creator	Lee, Jong-Soo Li, Rui Kim, Tae-Hwan Hong, Seung-Pyo Kim, Chul-Joong Kim, Jae-Hoon Park, Min-Eun Pathinayake, Prabuddha Son, Hwa-Young Sung, Moon-Hee Weeratunga, Prasanna Song, Man Tompkins, Stephen Chowdhury, Mohammed
topic	covid:efaa556b484fbcd9cc34832ffac53ef3e834e9c0#this
Source	PMC
abstract	To develop a safe and effective mucosal vaccine against pathogenic influenza viruses, we constructed recombinant Lactobacillus casei strains that express conserved matrix protein 2 with (pgsA-CTA1-sM2/L. casei) or without (pgsA-sM2/L. casei) cholera toxin subunit A1 (CTA1) on the surface. The surface localization of the fusion protein was verified by cellular fractionation analyses, flow cytometry and immunofluorescence microscopy. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA. However, the conjugation of cholera toxin subunit A1 induced more potent mucosal, humoral and cell-mediated immune responses. In a challenge test with 10 MLD(50) of A/EM/Korea/W149/06(H5N1), A/Puerto Rico/8/34(H1N1), A/Aquatic bird /Korea/W81/2005(H5N2), A/Aquatic bird/Korea/W44/2005(H7N3), and A/Chicken/Korea/116/2004(H9N2) viruses, the recombinant pgsA-CTA1-sM2/L. casei provided better protection against lethal challenges than pgsA-sM2/L. casei, pgsA/L. casei and PBS in mice. These results indicate that mucosal immunization with recombinant L. casei expressing CTA1-conjugated sM2 protein on its surface is an effective means of eliciting protective immune responses against diverse influenza subtypes.
has issue date	2014-04-08(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0094051
bibo:pmid	24714362
has license	cc-by
sha1sum (hex)	efaa556b484fbcd9cc34832ffac53ef3e834e9c0
schema:url	https://doi.org/10.1371/journal.pone.0094051
resource representing a document's title	Mucosal Vaccination with Recombinant Lactobacillus casei-Displayed CTA1-Conjugated Consensus Matrix Protein-2 (sM2) Induces Broad Protection against Divergent Influenza Subtypes in BALB/c Mice
has PubMed Central identifier	PMC3979752
has PubMed identifier	24714362
schema:publication	PLoS One
resource representing a document's body	covid:efaa556b484fbcd9cc34832ffac53ef3e834e9c0#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/efaa556b484fbcd9cc34832ffac53ef3e834e9c0
is schema:about of	named entity 'PROTECTION' named entity 'MLD' named entity 'high' named entity 'viruses' named entity 'immunofluorescence microscopy' named entity 'conserved' named entity 'Korea' named entity 'sM2' named entity 'CONJUGATION' named entity 'VERIFIED' named entity 'LACTOBACILLUS CASEI' named entity 'NASAL' named entity 'CTA1' named entity 'CHALLENGE TEST' named entity 'CELL' named entity 'FLOW CYTOMETRY' named entity 'LOCALIZATION' named entity 'IGA' named entity 'CHOLERA TOXIN' named entity '2F8' named entity 'MUCOSAL' named entity 'AQUATIC' named entity 'SERUM IMMUNOGLOBULIN G' named entity 'ORAL' named entity 'CONSERVED' named entity 'RICO' named entity 'INFLUENZA VIRUSES' named entity 'IMMUNE RESPONSES' named entity 'SM2' named entity 'MLD' named entity 'EFFECTIVE' named entity 'LACTOBACILLUS CASEI' named entity 'PGSA' named entity 'VIRUSES' named entity 'MATRIX PROTEIN 2' named entity 'MUCOSAL VACCINE' named entity 'HIGH' named entity 'FRACTIONATION' named entity 'SUBTYPES' named entity 'INFLUENZA' named entity 'DISPLAYED' named entity 'RECOMBINANT' named entity 'CONJUGATED' named entity 'BALB' named entity 'CONSENSUS' named entity 'CTA1' named entity 'RECOMBINANT' named entity 'FUSION PROTEIN' named entity 'PATHOGENIC' named entity 'MEDIATED' named entity 'PROTEIN ' named entity 'SUBUNIT' named entity 'MUCOSAL VACCINATION' named entity 'INOCULATIONS' named entity 'LEVELS' named entity 'INDUCED' named entity 'STRAINS' named entity 'SAFE' named entity 'EXPRESS' named entity 'BIRD' named entity 'CONSTRUCTED' named entity 'BROAD' named entity 'MICE' named entity 'MATRIX PROTEIN' named entity 'IMMUNOFLUORESCENCE MICROSCOPY' named entity 'SURFACE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software