About: Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL-mediated apoptosis

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About: Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL-mediated apoptosis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL-mediated apoptosis
Creator	Wang, Lin-Fa Zhou, Peng Bingham, John Green, Diane Mok, Lawrence Shiell, Brian Klein, Reuben Monaghan, Paul Tachedjian, Mary Todd, Shawn Boyd, Victoria Heesom, Kate Matthews, David Payne, Jean Marsh, Glenn Baker, Michelle Harper, Jennifer Wynne, James
source	Medline; PMC
abstract	BACKGROUND: Bats are a major reservoir of emerging infectious viruses. Many of these viruses are highly pathogenic to humans however bats remain asymptomatic. The mechanism by which bats control viral replication is unknown. Here we utilize an integrated approach of proteomics informed by transcriptomics to compare the response of immortalized bat and human cells following infection with the highly pathogenic bat-borne Hendra virus (HeV). RESULTS: The host response between the cell lines was significantly different at both the mRNA and protein levels. Human cells demonstrated minimal response eight hours post infection, followed by a global suppression of mRNA and protein abundance. Bat cells demonstrated a robust immune response eight hours post infection, which led to the up-regulation of apoptosis pathways, mediated through the tumor necrosis factor-related apoptosis inducing ligand (TRAIL). HeV sensitized bat cells to TRAIL-mediated apoptosis, by up-regulating death receptor transcripts. At 48 and 72 hours post infection, bat cells demonstrated a significant increase in apoptotic cell death. CONCLUSIONS: This is the first study to comprehensively compare the response of bat and human cells to a highly pathogenic zoonotic virus. An early induction of innate immune processes followed by apoptosis of virally infected bat cells highlights the possible involvement of programmed cell death in the host response. Our study shows for the first time a side-by-side high-throughput analysis of a dangerous zoonotic virus in cell lines derived from humans and the natural bat host. This enables a way to search for divergent mechanisms at a molecular level that may influence host pathogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-014-0532-x) contains supplementary material, which is available to authorized users.
has issue date	2014-11-15(xsd:dateTime)
bibo:doi	10.1186/s13059-014-0532-x
bibo:pmid	25398248
has license	cc-by
sha1sum (hex)	f00106cad50635bb15409ac6039b93b5af031565
schema:url	https://doi.org/10.1186/s13059-014-0532-x
resource representing a document's title	Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL-mediated apoptosis
has PubMed Central identifier	PMC4269970
has PubMed identifier	25398248
schema:publication	Genome Biol
resource representing a document's body	covid:f00106cad50635bb15409ac6039b93b5af031565#body_text
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