About: Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

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About: Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types
Creator	Gorgoglione, Bartolomeo Leaman, Douglas Pham, Loc Ringiesn, Jeffery Shepherd, Brian
Source	PMC
abstract	BACKGROUND: Infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) are highly contagious, pathogenic Novirhabdoviruses affecting fish and are thusly notifiable diseases with the World Organization for Animal Health. This study assessed the relative capacities of IHNV and VHSV genes to modulate host general transcription and explores the abilities of specific IHNV genes to interfere with the interferon pathway in heterogenous teleost cell-lines. METHODS: Optimized protocols allowed for efficient transient transfections in EPC, BF-2, RTG-2 and RTgill-W1 cell lines of plasmids encoding IHNV (M genogroup) and VHSV (-IVb genotype) genes, including N, P, M, G and NV. Their impact on general cellular transcription was measured 48 hours post transfection (hpt) with luciferase constructs driven by a modified β-Actin promoter (pCAG). Their modulation of the innate antiviral immune response was characterized 72 hpt, using luciferase constructs measuring rainbow trout Type I IFN or MX-1 promoter augmentation, upon MAVS co-transfection. RESULTS: M was generally confirmed as the strongest constitutive transcriptional suppressor while IHNV P, but not VHSV P, augmented constitutive transcription in fibroblastic cell types. Cell-specific effects were observed for viral G gene, with VHSV G exhibiting suppression of basal transcription in EPC and BF-2 but not in trout cells; while IHNV G was stimulatory in RTG-2, but inhibitory in RTgill-W1. NV consistently stimulated constitutive transcription, with higher augmentation patterns seen in fibroblastic compared to epithelial cells, and for IHNV NV compared to VHSV NV. The innate antiviral immune response, focusing on the IFN pathway, was silenced by IHNV M in all cell lines tested. IHNV N showed a dose-dependent suppression of type I IFN, but with minor effects on MX-1. IHNV P and G played minor IFN-inhibitory roles, consistent and dose-dependent only for G in rainbow trout cells. IHNV NV mediated a consistent stimulatory effect on either Type I IFN or MX-1, but much less pronounced in RTgill-W1. CONCLUSIONS: This study extends our understanding of Novirhabdoviruses-host interaction, showing differential innate immune responses in heterogenous cell types. Viral regulators of innate immune signaling are identified, either as dose-dependent suppressors (such as M and N) or stimulators (mainly NV), indicating novel targets for the design of more efficient vaccination strategies.
has issue date	2020-07-20(xsd:dateTime)
bibo:doi	10.1186/s12985-020-01372-4
has license	cc-by
sha1sum (hex)	f0a7d4797373675373b913f750e8dd467c31fee7
schema:url	https://doi.org/10.1186/s12985-020-01372-4
resource representing a document's title	Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types
has PubMed Central identifier	PMC7369537
schema:publication	Virol J
resource representing a document's body	covid:f0a7d4797373675373b913f750e8dd467c31fee7#body_text
is schema:about of	named entity 'notifiable' named entity 'Organization' named entity 'heterogenous' named entity 'augmentation' named entity 'transfection' named entity 'MX-1' named entity 'Methods' named entity 'capacities' named entity 'immune response' named entity 'transcription' named entity 'World' named entity 'promoter' named entity 'modulating' named entity 'luciferase' named entity 'viral hemorrhagic septicemia virus' named entity 'IHNV' named entity 'VHSV' named entity 'MAVS' named entity 'cell-lines' named entity 'IHNV' named entity 'VHSV' named entity 'teleost' named entity 'constitutive transcription' named entity 'rainbow trout' named entity 'IFN' named entity 'transfection' named entity 'cytotoxic effects' named entity 'plasmids' named entity 'MHC' named entity 'upregulated' named entity '0.01' named entity 'microplate reader' named entity 'viral infections' named entity 'viruses' named entity 'gene' named entity 'pathogens' named entity 'novirhabdovirus' named entity 'teleost' named entity 'teleost' named entity 'IFN' named entity 'rainbow trout' named entity 'western blot' named entity 'kDa' named entity 'antibody' named entity 'Cell lysates' named entity 'transfection' named entity 'IHNV' named entity 'plasmids' named entity 'VHSV' named entity 'statistically significant' named entity 'gene' named entity 'transcriptional' named entity 'SV40' named entity 'ISG' named entity 'luciferase' named entity 'β-Actin' named entity 'Phosphoprotein' named entity 'fibroblastic' named entity 'IHNV' named entity 'IFN' named entity 'constitutive transcription' named entity 'simian virus 40' named entity 'β-Actin' named entity 'Supp' named entity 'fibroblastic' named entity 'transfected' named entity 'Phosphoprotein' named entity 'β-Actin' named entity 'transfection' named entity 'US Government' named entity 'antiviral' named entity 'MAVS' named entity 'RNA-dependent RNA polymerase' named entity 'microplate reader' named entity 'IFN' named entity 'downregulation' named entity 'IHNV' named entity 'Glycoprotein' named entity 'ATCC'

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