About: Single Particle Tracking Assay to Study Coronavirus Membrane Fusion

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Single Particle Tracking Assay to Study Coronavirus Membrane Fusion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Single Particle Tracking Assay to Study Coronavirus Membrane Fusion
Creator	Daniel, Susan Costello, Deirdre
Source	Medline; PMC
abstract	Single particle tracking (SPT) of individual virion fusion with host cell membranes using total internal reflection microscopy (TIRFM) is a powerful technique for quantitatively characterizing virus–host interactions. One significant limitation of this assay to its wider use across many types of enveloped viruses, such as coronavirus, has been incorporating non-lipid receptors (proteins) into the supported lipid bilayers (SLBs) used to monitor membrane fusion. Here, we describe a method for incorporating a proteinaceous viral receptor, feline aminopeptidase N (fAPN), into SLBs using cell blebbing of mammalian cells expressing fAPN in the plasma membrane. This receptor binds feline coronavirus (FECV 1683). We describe how to carry out single particle tracking of FECV fusion in this SLB platform to obtain fusion kinetics.
has issue date	2014-12-18(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-2438-7_16
bibo:pmid	25720481
has license	no-cc
sha1sum (hex)	f16646d96608566be8cf15872a2f60fcfc3b3c6d
schema:url	https://doi.org/10.1007/978-1-4939-2438-7_16
resource representing a document's title	Single Particle Tracking Assay to Study Coronavirus Membrane Fusion
has PubMed Central identifier	PMC7123321
has PubMed identifier	25720481
schema:publication	Coronaviruses
resource representing a document's body	covid:f16646d96608566be8cf15872a2f60fcfc3b3c6d#body_text
is schema:about of	named entity 'fusion' named entity 'describe' named entity 'host' named entity 'proteins' named entity 'MEMBRANE FUSION' named entity 'PARTICLE TRACKING' named entity 'CELLS' named entity 'KINETICS' named entity 'BINDS' named entity 'AMINOPEPTIDASE N' named entity 'HOST' named entity 'HOW' named entity 'CARRY' named entity 'MONITOR' named entity 'PLATFORM' named entity 'POWERFUL' named entity 'LIPID' named entity 'SINGLE' named entity 'LIMITATION' named entity 'USING' named entity 'FELINE CORONAVIRUS' named entity 'MICROSCOPY' named entity 'SLB' named entity 'RECEPTOR' named entity 'CHAPTER' named entity 'MEMBRANE FUSION' named entity 'CORONAVIRUS' named entity 'CELL MEMBRANES' named entity 'USED' named entity 'HERE' named entity 'VIRUSES' named entity 'STUDY' named entity 'SINGLE' named entity 'VIRION' named entity 'TYPES' named entity 'CELL BLEBBING' named entity 'TECHNIQUE' named entity 'INDIVIDUAL' named entity 'SLBS' named entity 'HOST CELL' named entity 'VIRAL RECEPTOR' named entity 'ASSAY' named entity 'ACROSS' named entity 'FELINE' named entity 'ASSAY' named entity 'USE' named entity 'TOTAL' named entity 'VIRUS' named entity 'LIPID BILAYERS' named entity 'NON-' named entity 'FUSION' named entity 'CORONAVIRUS' named entity 'PARTICLE TRACKING' named entity 'ITS' named entity 'MAMMALIAN' named entity 'CANT' named entity 'IS A' named entity 'RECEPTORS' named entity 'OBTAIN' named entity 'PLASMA MEMBRANE' named entity 'INTERACTIONS' named entity 'DESCRIBE' named entity 'SUPPORTED' named entity 'INTERNAL' named entity 'METHOD' named entity 'PROTEINACEOUS' named entity 'incorporating' named entity 'fusion' named entity 'TIRFM' named entity 'describe' named entity 'fusion' named entity 'laser' named entity 'carry' named entity 'Single particle tracking' named entity 'interactions' named entity 'mammalian cells' named entity 'single particle tracking' named entity 'Single particle tracking' named entity 'coronavirus' named entity 'enveloped viruses'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software