About: Tissue Tropism and Target Cells of NSs-Deleted Rift Valley Fever Virus in Live Immunodeficient Mice

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About: Tissue Tropism and Target Cells of NSs-Deleted Rift Valley Fever Virus in Live Immunodeficient Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Tissue Tropism and Target Cells of NSs-Deleted Rift Valley Fever Virus in Live Immunodeficient Mice
Creator	Van Rooijen, Nico Guillemot, Laurent Hasan, Milena Montagutelli, Xavier Panthier, Jean-Jacques Agnè S Billecocq, Gory Jouvion, Gré Le Bouloy, Michè Line Gommet, Cé Ne Blanchet, Charlè Nia Zaverucha Do Valle, Tâ
topic	covid:f18afbc0a4621e7f13dcfbc67c85bf8dfe3b30ea#this
source	PMC
abstract	BACKGROUND: Rift Valley fever virus (RVFV) causes disease in livestock and humans. It can be transmitted by mosquitoes, inhalation or physical contact with the body fluids of infected animals. Severe clinical cases are characterized by acute hepatitis with hemorrhage, meningoencephalitis and/or retinitis. The dynamics of RVFV infection and the cell types infected in vivo are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: RVFV strains expressing humanized Renilla luciferase (hRLuc) or green fluorescent protein (GFP) were generated and inoculated to susceptible Ifnar1-deficient mice. We investigated the tissue tropism in these mice and the nature of the target cells in vivo using whole-organ imaging and flow cytometry. After intraperitoneal inoculation, hRLuc signal was observed primarily in the thymus, spleen and liver. Macrophages infiltrating various tissues, in particular the adipose tissue surrounding the pancreas also expressed the virus. The liver rapidly turned into the major luminescent organ and the mice succumbed to severe hepatitis. The brain remained weakly luminescent throughout infection. FACS analysis in RVFV-GFP-infected mice showed that the macrophages, dendritic cells and granulocytes were main target cells for RVFV. The crucial role of cells of the monocyte/macrophage/dendritic lineage during RVFV infection was confirmed by the slower viral dissemination, decrease in RVFV titers in blood, and prolonged survival of macrophage- and dendritic cell-depleted mice following treatment with clodronate liposomes. Upon dermal and nasal inoculations, the viral dissemination was primarily observed in the lymph node draining the injected ear and in the lungs respectively, with a significant increase in survival time. CONCLUSIONS/SIGNIFICANCE: These findings reveal the high levels of phagocytic cells harboring RVFV during viral infection in Ifnar1-deficient mice. They demonstrate that bioluminescent and fluorescent viruses can shed new light into the pathogenesis of RVFV infection.
has issue date	2011-12-06(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0001421
bibo:pmid	22163058
has license	cc-by
sha1sum (hex)	f18afbc0a4621e7f13dcfbc67c85bf8dfe3b30ea
schema:url	https://doi.org/10.1371/journal.pntd.0001421
resource representing a document's title	Tissue Tropism and Target Cells of NSs-Deleted Rift Valley Fever Virus in Live Immunodeficient Mice
has PubMed Central identifier	PMC3232203
has PubMed identifier	22163058
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:f18afbc0a4621e7f13dcfbc67c85bf8dfe3b30ea#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/f18afbc0a4621e7f13dcfbc67c85bf8dfe3b30ea
is schema:about of	named entity 'infected' named entity 'retinitis' covid:arg/f18afbc0a4621e7f13dcfbc67c85bf8dfe3b30ea named entity 'physical' named entity 'contact' named entity 'body fluids' named entity 'infected' named entity 'Rift Valley' named entity 'livestock' named entity 'RVFV' named entity 'acute hepatitis' named entity 'retinitis' named entity 'body fluids' named entity 'transmitted by mosquitoes' named entity 'meningoencephalitis' named entity 'mosquito' named entity 'ruminants' named entity 'luciferase' named entity 'inoculation' named entity 'RVFV' named entity 'apoptosis' named entity 'pancreas' named entity 'phagocytic cells' named entity 'Phagocytic cells' named entity 'macrophages' named entity 'CD11b' named entity 'intradermally' named entity 'GFP' named entity 'RVFV' named entity 'CLL' named entity 'Alexa Fluor' named entity 'lung' named entity 'infection' named entity 'infection' named entity 'airborne transmission' named entity 'Infection' named entity 'phagocytic cells' named entity 'Ifnar1' named entity 'hepatitis' named entity 'viral replication' named entity 'RVFV' named entity 'antibodies' named entity 'spleen' named entity 'body fluids' named entity 'clodronate' named entity 'liver' named entity 'viral genome' named entity 'CLL' named entity 'nucleocapsid' named entity 'infection' named entity 'Ifnar1' named entity 'hematopoietic cell' named entity 'firefly luciferase' named entity 'macrophages' named entity 'rat' named entity 'infection' named entity 'Vero E6' named entity 'peritoneum' named entity '1:9' named entity 'apoptosis' named entity 'intestine' named entity 'intranasally' named entity 'infection' named entity 'abdomen' named entity 'CD3' named entity 'clodronate' named entity 'intraperitoneally' named entity 'plasmid' named entity 'Pacific Blue'

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